Method for separating and purifying L-ornithine by using simulated moving bed

Abstract

The invention belongs to the field of biological pharmaceutics, and discloses a method for separating and purifying L-ornithine by using a simulated moving bed. The method comprises the following steps: firstly, removing bacteria and solid substances from L-ornithine fermentation solution by using ultra-filtration; secondly, feeding the solution into the simulated moving bed, adsorbing the solution, washing the impurities, eluting the L-ornithine, collecting the elution and regenerating a column; and finally, concentrating and decolorizing the collected elution, adjusting the pH of the elution to between 4.5 and 5.0 with hydrochloric acid, crystallizing the elution, and drying the crystal to obtain L-ornithine hydrochloride crystal. The L-ornithine separated and purified by using the method has the advantages of high yield, high purity, low cost, environmental protection, and suitability for industrialized production.

Description

technical field [0001] The invention belongs to the field of biopharmaceuticals, and relates to a method for separating and purifying L-ornithine from fermentation liquid by using a simulated moving bed. Background technique [0002] L-ornithine is an important non-protein constituent amino acid and a precursor for the biosynthesis of L-arginine. L-ornithine plays an extraordinary role in the human body, the formation of urea must have the participation of L-ornithine, the lack of L-ornithine may bring danger to life. Now L-ornithine is widely used in medicine and food industry, mainly used in the treatment of acute and chronic hepatitis, hepatic encephalopathy and other diseases (GB1020492, 1966), the recovery of the patient's body, sports drinks, and the prevention and treatment of benign cysts and malignant tumors (CA2404275, 2001) and so on. [0003] At present, domestic and foreign reports on the preparation methods of L-ornithine mainly include chemical methods, enzy...

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Problems solved by technology

The chemical method is mainly to synthesize DL-ornithine by alkaline hydrolysis of L-arginine and acrylic acid and sodium cyanide. In addition, the chemical method requires a large amount of organic solvents, which seriously pollutes the environment; the enzymatic conversion method uses L-arginine as raw material, ferments Cultivate cells and catalyze the conversion into L-ornithine by the enzymes produced; the production of L-ornithine by microbial fermentation mainly uses low-cost raw materials for submerged liquid fermentation to directly produce L-ornithine. The fermentation level is relatively lower than that of foreign countries, but with the in-depth research on strains and process technology, its fermentation output will continue to increase

[0004] There are many impurities in the L-ornithine fermentation broth obtained by the above method, and it is difficult to separate and purify

Some literatures have reported utilizing traditional ion exchange technology to separate L-ornithine in fermentation broth. The yield of L-ornithine separated and purified by this method is low, water and eluent consumption are large, and resin regeneration needs to consume a large amount of Acid and alkali, so that the cost of the whole separation and purification is higher

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