Synergistic composition for preventing and curing fruit and vegetable diseases
The technology of a composition and fungicide composition, applied in the field of pesticides, can solve problems such as unreported and unsatisfactory treatment effects, and achieve the effects of reducing stress, delaying the occurrence and development of pathogenic bacteria resistance, and saving dosage
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Embodiment 1
[0014] Bioassay Example 1: Toxicity determination of bromothalonil and dicyanoanthraquinone complex on apple ringworm
[0015] The indoor toxicity of the mixture of bromothalonil and dicyanoanthraquinone to apple ringworm was studied by the method of mycelium growth rate. The experimental method used Sun Yunpei's method to calculate the co-toxicity coefficient (CTC) to evaluate the mixed effect. CTC When the value is greater than 120, it is considered that the mixture has a synergistic effect, when it is less than 80, it is considered an antagonistic effect, and when it is between 80 and 120, it is considered to have an additive effect.
[0016] The specific method is as follows:
[0017] Take the prepared medicinal solution and put it in 100ml of 50-60℃ PDA sterilized medium, shake well, pour it into a sterilized petri dish with a diameter of 60mm, after cooling and solidifying, transplant the bacteria cakes with uniform growth and a diameter of 4mm, The sterilized medium ad...
Embodiment 2
[0028] Bioassay Example 2: Toxicity determination of bromothalonil and dicyanoanthraquinone complex to cucumber downy mildew
[0029] The indoor toxicity of bromothalonil and dicyananthraquinone to cucumber downy mildew was determined by plate leaf method. Test method: Put the cucumber leaves in a petri dish for moisturizing treatment, spray the pesticides according to the designed doses, inoculate cucumber downy mildew bacteria after 24 hours, and culture them in an incubator with moisture, investigate the control effect after 7 days, and use the disease index Calculate the control effect, use DPS data processing software for statistical analysis, calculate the EC50 of each agent, and use Sun Yunpei's method to calculate the co-toxicity coefficient (CTC) to evaluate the mixed use effect. The test results show that the combination of bromothalonil and dicyanoanthraquinone has good indoor toxicity to cucumber downy mildew, and the CTC values of all the ratios are more than 12...
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