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Extraction method of total saponin of Radix Ilicis Asprellae and quality detection method thereof

A quality detection method and an extraction method technology, applied in the quality detection field of Gangmei total saponins, can solve the problems such as the blank quality detection method of Gangmei total saponins

Active Publication Date: 2010-07-07
CHINA RESOURCES SANJIU MEDICAL & PHARMA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In addition, the prior art is still blank for the quality detection method of the extracted total saponins of Plum

Method used

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  • Extraction method of total saponin of Radix Ilicis Asprellae and quality detection method thereof
  • Extraction method of total saponin of Radix Ilicis Asprellae and quality detection method thereof
  • Extraction method of total saponin of Radix Ilicis Asprellae and quality detection method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1 The extraction process of the total saponins of Prunus officinalis

[0044] 1. Orthogonal experimental design

[0045] The retention rates of total saponins and ilexoside X X IX were used as indicators respectively, and an orthogonal experiment design with four factors and three levels was adopted. Weigh 20 g of Gangmei medicinal material, carry out the experiment according to the orthogonal design in Table 1, combine the filtrates, and measure the volume.

[0046] Table 1 Factor level table of orthogonal experiment for the extraction process of Gangmei medicinal materials

[0047]

[0048] 2. Preparation of the test solution

[0049] 2.1 Preparation of total saponins test solution

[0050] Accurately measure the appropriate amount of medicinal liquid under each orthogonal test item, concentrate under reduced pressure until there is no alcohol smell, dilute with water until the loading concentration is 0.2mg of crude drug / ml, accurately measure ...

Embodiment 2

[0073] Example 2 AB-8 Macroporous Adsorption Resin Refining Process of Gangmei Total Saponins Extract

[0074] 1. Investigation of sample concentration

[0075] 1.1 Preparation of Gangmei Extract and Determination of Gangmei Total Saponins in Extract

[0076] Get 905g of Gangmei medicinal material coarse powder (batch number 20080401 of Chinese medicine beverage factory of Guangzhou medicinal material company), add 8 times of 60% ethanol, heat and reflux for 1.5 hours, filter, add 6 times of 60% ethanol for filter residue, heat and reflux for 1 hour, filter, combine the filtrates, and measure the volume to obtain 11020ml of extract, which is set aside. The total saponin content was determined.

[0077] 1.2 Investigation of the retention rate of total saponins at different loading concentrations (centrifugation, static adsorption)

[0078] Measure 608.8ml of Gangmei extract (equivalent to containing 50g of crude drug), recover ethanol under reduced pressure, concentrate to 1...

Embodiment 3

[0133] Example 3 Verification of the purification process of Gangmei total saponins extract AB-8 macroporous resin

[0134] 1. Preparation of Gangmei Extract and Determination of Gangmei Total Saponins and Ilexoside X X IX in the Extract

[0135] According to the proposed conditions, get 250g of Gangmei medicinal material coarse powder (Chinese medicine beverage factory of Guangzhou medicinal material company, batch number: 20080401), add 8 times of 60% ethanol, heat and reflux for 1.5 hours, filter, and add 6 times of 60% ethanol to the filter residue. % ethanol, heated to reflux for 1 hour, filtered, combined the filtrates, and measured the volume to obtain 3016ml of extract, which was set aside. Determination of the content of total saponins and ilexosideX X IX of Gangmei.

[0136] 2. Verification of the refining process of Gangmei AB-8 macroporous adsorption resin

[0137] According to the maximum loading amount of Gangmei medicinal liquid macroporous resin determined in...

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Abstract

The invention discloses an extraction method of total saponin of Radix Ilicis Asprellae and a quality detection method thereof, and the extraction method comprises the following steps: taking a medicinal material of Radix Ilicis Asprellae root or stem, adding 4-12 times volume of 60% ethanol, extracting for 1.5-2h, adding 2-10 times volume of 60% ethanol, extracting for 1.0-1.5h, filtering and merging filtrate; concentrating under reduced pressure until no alcohol taste exists, adding water, diluting crude drug with the loading concentration of 0.1-0.5g / ml, and loading a pre-treated AB-8 macroporous resin column; washing the resin column to be cleared by water, and eluting by 60-80% of ethanol; and collecting eluent of 60-80% ethanol and concentrating under reduced pressure, drying to obtain the total saponin of Radix Ilicis Asprellae. The quality detection method comprises the steps of TLC chromatograph identification and content determination of ilexoside X XIX in the total saponin of Radix Ilicis Asprellae. By using the refined technology of the invention, the retention rates of the total saponin of Radix Ilicis Asprellae and f ilexoside X XIX are improved greatly, and the obtained extract has higher purity; the obtained total saponin of Radix Ilicis Asprellae has the functions of inflammation resistance, analgesis, koortswerend, antivirus and the like; by using the characteristic saponin ilexoside X XIX as a reference sample, the accuracy is higher and the result has high reliability; and the quality detection method of the total saponin of Radix Ilicis Asprellae extract can provide a reference for quality control of the total saponin of Radix Ilicis Asprellae extract.

Description

technical field [0001] The invention relates to a method and application for extracting total saponins of Gangmei from medicinal materials of Gangmei, and also relates to a quality detection method of total saponins of Gangmei. Background technique [0002] Gangmei is the dry root and stem of Ilex holly, which is recorded in the "Guangdong Provincial Standards of Chinese Medicinal Materials" (Volume 1) Guangdong D / WS-058-2003. It is mainly produced in Guangdong, Guangxi and other places. It is a traditional Chinese medicine in Lingnan. Functions and indications are heat-clearing and detoxifying, promoting body fluid and quenching thirst, relieving sore throat and reducing swelling, dispelling blood stasis and relieving pain; used for cold and fever, cough due to lung heat, thirsty wounds due to febrile disease, sore throat, bruises and pain. It mainly contains triterpene saponins, lactones (coumarin) and a small amount of alkaloids. [0003] Macroporous adsorption resin is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61K8/97A61P29/00A61P31/16A61Q11/00G01N30/02G01N30/90A23G4/06A23L2/38
Inventor 张军张晓琦李润美汤毅叶文才赖小平
Owner CHINA RESOURCES SANJIU MEDICAL & PHARMA
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