Method for removing heavy metal cadmium from water solution
An aqueous solution, heavy metal technology, applied in chemical instruments and methods, water pollutants, water/sewage treatment, etc.
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Embodiment 1
[0013] After the preserved Saccharomyces ruckeri was activated on a YEPD solid slope at a constant temperature of 28°C, it was prepared into commonly used liquid seeds, and the liquid seeds were inoculated into the YEPD fermentation medium with an inoculum volume of 5%, a liquid volume of 50%, and a constant temperature of 30 ℃, rotating speed 180rpm, culture for 12h, centrifuge the fermented liquid at 4200rpm for 10min, collect the cells and wash with distilled water, after freeze-drying, grind and sieve to prepare the dry yeast powder of Luxie. Put the dry powder of Luxie yeast into the solution containing 10 mg / L cadmium according to the amount of the bacterial cell concentration after adding the dry yeast powder of Luxie, adjust the pH value of the liquid containing cadmium to 5.0, room temperature, shake occasionally, and absorb for 30 minutes; After standing still for 60 minutes, the Saccharomyces ruckeri with adsorbed cadmium was separated and removed.
Embodiment 2
[0015] After the preserved Saccharomyces ruckeri was activated on a wort solid slope at a constant temperature of 30°C, the wort liquid seeds were prepared, and the liquid seeds were inoculated into the YEPD fermentation medium with an inoculum size of 7%, a liquid content of 40%, and a constant temperature 28°C, rotating speed 160rpm, incubate for 22h, centrifuge the fermentation broth at 3000rpm for 20min, collect the cells and wash with distilled water, freeze-dry, grind and sieve, and prepare into Luxie yeast dry powder. According to the amount that the bacterial cell concentration reaches 4g / L after adding the Luxie yeast dry powder, put the Luxie yeast dry powder into the solution containing 50mg / L cadmium, adjust the pH value of the cadmium-containing liquid to 6.0, the temperature is 30°C, and the shaking frequency is 100rpm. Adsorb for 20 minutes; after standing still for 120 minutes, separate and remove the Luxie yeast adsorbed with cadmium.
Embodiment 3
[0017] After the preserved Saccharomyces ruckeri strains were activated on a wort solid slope at a constant temperature of 29°C, they were prepared into commonly used YEPD liquid seeds, and the liquid seeds were inoculated into the YEPD fermentation medium with an inoculation amount of 6% and a liquid filling amount of 20%. Constant temperature at 35°C, 150rpm, culture for 24h, centrifuge the fermentation broth at 3500rpm for 30min, collect the cells and wash with distilled water, freeze-dry, grind and sieve, and prepare into Luxie yeast dry powder; after adding Luxie yeast dry powder, the bacterial cell concentration reaches The amount of 4.5g / L, put the dry powder of Luxie yeast into the solution containing 100mg / L cadmium, adjust the pH value of the cadmium-containing liquid to 7.0, the temperature is 10°C, the oscillation frequency is 60rpm, absorb for 20min, and after standing still for 180min, separate and remove the adsorbed Cadmium of ruckeri.
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