Method for producing high-lysine fermented feed through fermentation of vinegar residue using two-step method
A high-lysine, fermented feed technology, applied in animal feed, animal feed, application, etc., can solve the problems of unbalanced amino acid composition, difficult comprehensive utilization, unfavorable environmental protection, etc., to improve amino acid composition and intestinal microflora. Ecology, the effect of improving the added value of products
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Embodiment 1
[0019] A method for producing high-lysine fermented feed by fermenting vinegar grains in two steps, comprising the following steps:
[0020] (1) Prepare potato culture medium, take 20% of potatoes by mass, 2% of sucrose, and the balance is water, and sterilize at a temperature of 121°C for 13 minutes; inoculate Aspergillus niger strains into potato culture medium to make the The Aspergillus niger species accounted for 2% of the quality of the potato culture medium, cultivated under ventilated conditions for 32 hours at 28°C;
[0021] (2) prepare lysine seed culture medium, take glucose 2.0% by mass, (NH 4 ) 2 SO 4 0.4%, urea 0.1%, K 2 HPO 4 0.1%, corn steep liquor 2%, soybean cake hydrolase 2%, MgSO 4 0.025%, the balance is water, adjust the pH to be 7.0, and sterilize at 0.1Mpa for 15min; Corynebacterium glutamicum bacterial classification is inoculated in the lysine seed culture medium, makes described Corynebacterium glutamicum bacterial classification occupy lysine ...
Embodiment 2
[0026] A method for producing high-lysine fermented feed by fermenting vinegar grains in two steps, comprising the following steps:
[0027] (1) Prepare potato culture medium, take by weight 20% of potatoes, 2% of sucrose, and the balance is water, and sterilize at 121°C for 12 minutes; inoculate Aspergillus niger strains into potato culture medium, and make the The above-mentioned Aspergillus niger species accounted for 5% of the quality of the potato culture medium, cultivated under ventilated conditions for 18 hours at 35°C;
[0028] (2) prepare lysine seed culture medium, take glucose 2.0% by mass, (NH 4 ) 2 SO 4 0.4%, urea 0.1%, K 2 HPO 4 0.1%, corn steep liquor 2%, soybean cake hydrolase 2%, MgSO 4 0.025%, the balance is water, adjust the pH to be 7.0, and sterilize at 0.1Mpa for 15min; Corynebacterium glutamicum bacterial classification is inoculated in the lysine seed culture medium, makes described Corynebacterium glutamicum bacterial classification occupy lysin...
Embodiment 3
[0033] A method for producing high-lysine fermented feed by fermenting vinegar grains in two steps, comprising the following steps:
[0034] (1) Prepare potato culture medium, take by weight 20% of potatoes, 2% of sucrose, and the balance is water, and sterilize at 121°C for 10 minutes; inoculate Aspergillus niger strains into potato culture medium to make the The above-mentioned Aspergillus niger species accounted for 3% of the quality of the potato culture medium, cultivated under ventilated conditions for 48 hours at 25°C;
[0035] (2) prepare lysine seed culture medium, take glucose 2.0% by mass, (NH 4 ) 2 SO 4 0.4%, urea 0.1%, K 2 HPO 40.1%, corn steep liquor 2%, soybean cake hydrolase 2%, MgSO 4 0.025%, the balance is water, adjust the pH to be 7.0, and sterilize at 0.1Mpa for 15min; Corynebacterium glutamicum bacterial classification is inoculated in the lysine seed culture medium, makes described Corynebacterium glutamicum bacterial classification occupy lysine ...
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