Method for detecting instability of radiation-induced genome

Abstract

The invention relates to a genome detection method, in particular relating to a method for detecting the instability of radiation-induced genome. The detection method of the invention comprises the following steps: irradiating cells for the first time, measuring the SCGE and micronucleus incidence rate of the cells; and inheriting 25 generations of cells of each viable dose point after irradiation, measuring the SCGE and micronucleus incidence rate of the cells; secondarily irradiating the 26th or following passage cells of each viable dose point after irradiation, measuring the SCGE and micronucleus incidence rate of the cells once again, thus the fact that secondary irradiation can induce the instability of genome is obvious seen by comparing SCGE and the micronucleus incidence rate, and cells show obvious expansion damage effect. By adopting the method described in the invention, the instability of genome can be directly detected, and the defect that the instability of genome is difficult to be observed under conventional experiment conditions as the observation time required by traditional direct detection is long and the incidence rate is too low is overcome.

Description

technical field [0001] The invention relates to the technical field of genome detection methods, in particular to a detection method for radiation-induced genome instability. Background technique [0002] Genome instability means that the genome is in a damage-susceptible state, which can amplify and accumulate the occurrence of other damage. The final genetic change is reflected in the genome damage that persists for many generations after cell irradiation, while the irradiated cells themselves are not in a certain distorted form. Therefore, it is difficult to directly verify genomic instability using some conventional experimental methods (such as clone survival rate, single-cell gel electrophoresis SCGE, and micronucleus incidence), because some conventional methods can only be used when the damage has accumulated enough. Experimental means to detect the occurrence of genomic instability. [0003] Genome instability is a hot topic in current research, especially when it...

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Problems solved by technology

Therefore, it is difficult to directly verify genomic instability using some conventional experimental methods (such as clone survival rate, single-cell gel electrophoresis SCGE, and micronucleus incidence), because some conventional methods can only be used when the damage has accumulated enough. Experimental means to detect the occurrence of genomic instability

[0003] Genome instability is a hot topic in current research, especially when it is recognized that genome instability may be a crucial step in the multi-stage carcinogenesis theory, so the detection of genome instability has become an important research method, but the existing The above-mentioned shortcomings of the technology make it particularly necessary to rely on other methods to amplify the effects of genomic instability so that we can detect them under routine experimental conditions

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