Corn silk polysaccharide separation and decoloring method
A corn filigree and polysaccharide technology, applied in the field of plant polysaccharide separation and decolorization, can solve the problems of different effects, low pigment removal rate, difficult removal of activated carbon, etc., and achieves the effect of maintaining color and luster, simple process and remarkable effect.
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Embodiment 1
[0013] Grind the cleaned and dried corn filaments, weigh 50 g of the filament powder, add cellulase (enzyme activity 2000 units) of 1.0% of the filament mass, add distilled water at a ratio of 1:10, adjust the pH to 4.5, and place in a constant temperature water bath at 45°C. Stir for enzymatic hydrolysis for 5h. The filigree polysaccharide extract after enzymolysis is filtered with nylon mesh or gauze, centrifuged, the precipitate is discarded, the supernatant is taken, and vacuum concentrated to 1 / 3 volume by a rotary evaporator. The Sevage method is used to remove protein, and the specific operation is to mix the supernatant in the previous step: deproteinization reagent (chloroform: n-butanol = 5: 1) = 5: 1 (volume ratio), and centrifuge at 3500r / min for 10min. Take the supernatant. Add powdered activated carbon to the polysaccharide solution after deproteinization. The amount of activated carbon added is 0.08g / ml (polysaccharide solution after deproteinization). After mi...
Embodiment 2
[0018] The cleaned and dried corn filaments were pulverized, and 100 g of quantitative filament powder was weighed, cellulase (enzyme activity 1500) with a mass of 1.2% of the filaments was added, and distilled water was added in a ratio of 1:12.5. Adjust the pH value to 5.0 and place in a constant temperature water bath at 45°C for 4.5h. Filtrate the filigree polysaccharide extract with nylon mesh or gauze, centrifuge, discard the precipitate, take the supernatant, and use a rotary evaporator to vacuum concentrate to 1 / 3.5 volume. The Sevage method is used to remove protein, and the specific operation is to mix the supernatant in the previous step: deproteinization reagent (chloroform: n-butanol = 5: 1) = 5: 1 (volume ratio), and centrifuge at 4000r / min for 9min. Take the supernatant. Add powdered activated carbon to the polysaccharide solution after deproteinization. The amount of activated carbon added is 0.10g / ml (polysaccharide solution after deproteinization). After mix...
Embodiment 3
[0023] Crush the washed and dried corn filaments, weigh 200 g filament powder, add cellulase (enzyme activity 1000 units) with 1.5% filament mass, add distilled water at a ratio of 1:15, adjust the pH value to 5.5, and bathe in a constant temperature water bath at 45°C for 4 hours. Filtrate the filigree polysaccharide extract with nylon mesh or gauze, centrifuge, discard the precipitate, take the supernatant, and use a rotary evaporator to vacuum concentrate to 1 / 4 volume. The Sevage method is used to remove protein, and the specific operation is to mix the supernatant in the previous step: deproteinization reagent (chloroform: n-butanol = 5: 1) = 6: 1 (volume ratio), and centrifuge at 4500r / min for 8min. Take the supernatant. Add powdered activated carbon to the polysaccharide solution after deproteinization. The amount of activated carbon added is 0.12g / ml (polysaccharide solution after deproteinization). After mixing evenly, put it in a constant temperature water bath at 45...
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