Digital holographic microscopic imaging method based on surface plasma resonance and microscope

Abstract

The invention discloses a digital holographic microscopic imaging method based on surface plasma resonance and a microscope. In the microscopic imaging method, a linear polarization laser beam is expanded and collimated, and then is split into two beams, wherein one is a reference beam, the other enters a prism and is reflected by the prism surface provided with a metallic film to be an object beam; a sample to be tested is placed on the prism surface plated with the metallic film; after passing a combiner, the object beam and the reference beam enter a camera head to obtain a hologram and simultaneously obtain the strength allocation plan and phase allocation plan on the surface of the sample to be tested by a digital holographic reconstruction algorithm. The invention provides larger flexibility for selecting an incident angle, thereby having greater superiority. The surface image of a cell film, which is displayed by the phase microscopic image and the strength microscopic image obtained from the hologram, can not be obtained by the traditional microscope, therefore, the microscope of the invention has wide application prospect.

Description

technical field [0001] The invention relates to a digital holographic microscopic imaging technology based on surface plasmon resonance effect, which belongs to the field of microscopic imaging technology. Background technique [0002] Digital Holography (Digital Holography) is the product of the combination of ordinary holography, computer technology, and electronic imaging technology. The recording process of digital holography and ordinary holography is basically the same, the difference is that electronic imaging devices such as CCD cameras and CMOS cameras are used instead of holographic dry plates to record holograms, and the recorded holograms are stored in computers; the reproduction of digital holography The process is completely different from ordinary holography. It uses digital calculation methods to digitally reproduce the hologram stored in the computer. Compared with ordinary holography, it has the following obvious advantages: (1) Since the electronic imagin...

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Problems solved by technology

But this kind of total internal reflection digital holographic microscope has some limitations

For example, the difference in refractive index between the biological sample and the glass prism used to produce total internal reflection is about 0.1 to 0.4, which may make some local areas of the sample surface satisfy the condition of total internal reflection while others satisfy the same incident angle. The local area does not meet the total internal reflection condition, so it is usually necessary to set a larger incident angle so that all local areas meet the total internal reflection condition, which brings great inconvenience to digital holographic microscopic imaging, mainly in Due to the large reflection angle, the angle between the normal line of the reflection interface and the imaging optical axis is also large, resulting in a large compression distortion in one direction of the image plane

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