Primers and diagnostic methods for diagnosing mulberry bacterial wilt
A technique for bacterial wilt, which is applied in the field of primers and diagnosis of mulberry bacterial wilt by PCR method, can solve the problems of high primer specificity and long detection cycle, and achieve high primer specificity, short detection cycle and high sensitivity and high accuracy
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[0023] Primer synthesis
[0024] By comparing the mulberry bacterial wilt pathogen (Enterobacter morus) L1, L2, L3, L4 and 7 international standard strains (the international standard numbers are respectively Enterobacter cloacae subsp.dissolvens LMG2683 T , E. cancerogenus LMG2693 T , E. pyrinus LMG22970 T , E.turicensis LMG23730 T , E. helveticus LMG23732 T , E. nimipressuralisLMG10245 T and E.agglomerans LMG2557 T ) rpoB sequence, such as Figure 4 As shown, two specific regions (gray) of the pathogen of Mulberry bacterial wilt were found. According to the two specific regions, a pair of primers were obtained by using alignment software (ClustalW) and primer synthesis software (Primer5). The upstream primer (EMrpobF) is 5'-GCCAAGCCGATTTCTGGAGCA and the downstream primer (EMrpobR) is 5'-GCATACAGGTTAGCTTTGC, through which a DNA fragment with a size of about 300bp can be specifically amplified. The above primers can be synthesized by conventional methods, and the primer...
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