Production method for preparing inulase

A production method and a technology for inulinase, which are applied in the field of preparing inulinase, can solve the problems of high application cost, low yield of inulinase of enzyme-producing strains, restricting the application of fructooligosaccharides and high fructose syrup, and the like. Simple, enhanced permeability, stable and reliable production process

Inactive Publication Date: 2010-06-02
HENAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The inulinase yield of enzyme-producing strains is low, resulting in high application costs, which r...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1: A kind of production method of preparing inulinase

[0031] Preparation of spore suspension: inoculate Aspergillus niger species on the slant medium, the composition of the slant medium is (g / L): peptone 10, inulin 40 and agar 20, and the pH value of the slant medium is a natural value. The inoculated slant medium was placed in an incubator at 28° C. for constant temperature cultivation for 3 days to generate spores, and after the spores were taken out, 0.85% sterile saline was added to wash the spores to obtain a spore suspension. Then dilute the spore suspension with 0.85% sterile saline until the number of spores per milliliter of liquid is 10 7 spare.

[0032] The number of spores per milliliter of liquid is 10 7 Individual detection methods can be measured by plate colony counting method.

[0033] Preparation of liquid seeds: the composition of the liquid seed medium is (g / L): yeast powder 15, peptone 15 and inulin 40, and the pH value of the liqui...

Embodiment 2

[0036] Embodiment 2: A kind of production method of preparing inulinase

[0037] Preparation of spore suspension: inoculate Aspergillus niger species on the slant medium, the composition of the slant medium is (g / L): peptone 10, inulin 40 and agar 20, and the pH value of the slant medium is a natural value. The inoculated slant medium was placed in an incubator at 28° C. for constant temperature cultivation for 3 days to generate spores, and after the spores were taken out, 0.85% sterile saline was added to wash the spores to obtain a spore suspension. Then dilute the spore suspension with 0.85% sterile saline until the number of spores per milliliter of liquid is 10 7 spare.

[0038] The number of spores per milliliter of liquid is 10 7 Individual detection methods can be measured by plate colony counting method.

[0039] Preparation of liquid seeds: the composition of the liquid seed medium is (g / L): yeast powder 17, peptone 17 and inulin 35, and the pH value of the liqui...

Embodiment 3

[0042] Embodiment 3: A kind of production method of preparing inulinase

[0043] Preparation of spore suspension: inoculate Aspergillus niger species on the slant medium, the composition of the slant medium is (g / L): peptone 10, inulin 40 and agar 20, and the pH value of the slant medium is a natural value. The inoculated slant culture medium was placed in an incubator at 28° C. for 4 days to generate spores. After the spores were taken out, 0.85% sterile saline was added to wash the spores to obtain a spore suspension. Then dilute the spore suspension with 0.85% sterile saline until the number of spores per milliliter of liquid is 10 7 spare.

[0044] The number of spores per milliliter of liquid is 10 7 Individual detection methods can be measured by plate colony counting method.

[0045] Preparation of liquid seeds: the composition of the liquid seed culture medium is (g / L): yeast powder 10, peptone 10 and inulin 50, and the pH value of the liquid seed culture medium is ...

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Abstract

The invention relates to a production method for preparing inulase. The method comprises the following four steps of: the preparation of a spore suspension, the preparation of liquid seeds, the preparation of an aspergillus niger fermentation liquor and the preparation of an inulase product. The method specifically comprises the following steps of: inoculating aspergillus niger stains on a slant culture medium to culture the spore suspension; inoculating the spore suspension into a liquid seed culture medium at a temperature of 28-30 DEG C to culture so as to obtain the liquid seeds; inoculating the liquid seeds into an aspergillus niger fermentation medium to ferment according to an inoculation quantity of 3-7 percent in a volume ratio under an aseptic condition; within a period of time of 0-60h under a certain fermentation condition, opening an ultrasonic transmitting device to carry out ultrasonic irradiation culture on the aspergillus niger fermentation medium and the liquid seeds; after the ultrasonic irradiation culture, continuously carrying out ultrasonic irradiation culture to obtain the aspergillus niger fermentation liquor; precipitating inulase from the aspergillus niger fermentation liquor by adopting a salting-out method; and filtering waste liquid through a filter press and drying a filter cake to obtain an inulase product. The production method is simple and stable, saves the production cost, and improves the output of the inulase.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation, and in particular relates to a production method for preparing inulinase. Background technique [0002] Inulinase, scientific name (β-2, 1-D-fructanase), also known as (β-fructanase), or [2, 1-D-fructanase (EC 3.2.1 )]. It is a polysaccharide hydrolase that can hydrolyze the fructosidic bonds of (β-2,1-D-fructan). [0003] At present, the application prospect of inulinase is mainly reflected in three aspects: [0004] 1) Using inulinase to hydrolyze inulin to produce high fructose syrup. Because high fructose syrup is cheap, sweet, refreshing, high osmotic pressure, good preservation effect, low calorific value, not easy to cause dental caries, and can be used by diabetics, it is widely used in the food and pharmaceutical industries in the United States, Japan and other developed countries . Compared with the traditional method of producing fructose by using starch as raw mat...

Claims

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Application Information

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IPC IPC(8): C12N9/24C12R1/685
Inventor 罗登林钟先锋李佩艳辛莉李爱江袁海丽徐宝成刘建学许威王佳莹
Owner HENAN UNIV OF SCI & TECH
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