Composition of banana oligosaccharide and dietary fibre, preparation method and application thereof
A dietary fiber and soluble dietary fiber technology, which is applied in the field of functional food and its preparation, its preparation, banana oligosaccharides and dietary fiber composition, can solve the problems of unclear mechanism of action, backward processing technology, and no research reports. , to achieve the effect of preventing colorectal cancer, low cost, and simple preparation process
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Embodiment 1
[0036] Embodiment 1 prepares composition according to the following method
[0037] (1) Peel the bananas; beat the pulp for 2.5 minutes, extract with water, the weight-to-volume ratio of the material to liquid is 1g: 1ml, the temperature is 60°C, the extraction time is 50min, and the extraction is 4 times; the 4 extractions are combined, and the pomace is placed separately , the extract was filtered and concentrated in vacuum, precipitated with 4 times the volume of the extract in absolute ethanol, centrifuged at 3500 rpm for 14 minutes, collected the precipitate, and vacuum freeze-dried at 50Pa to 80Pa for 4 hours to obtain the banana oligosaccharide product;
[0038] (2) The remaining pomace after extraction of banana sugar liquid is used for enzymatic hydrolysis to prepare banana soluble and insoluble dietary fiber:
[0039] Step A: Add amylase to the pomace, the amount of amylase is 3.6% of the pomace weight, the action temperature is 100°C, add calcium chloride to make th...
Embodiment 2
[0044] (1) Peel the bananas; beat the pulp for 3 minutes, extract with water, the weight-to-volume ratio of the material to liquid is 1g: 1ml, the temperature is 50°C, the extraction time is 60min, and the extraction is performed 4 times; After conventional suction filtration and vacuum concentration, the extract was precipitated with 4 times the volume of the extract in absolute ethanol, centrifuged at 4500 rpm for 12 minutes, the precipitate was collected, and vacuum freeze-dried at 60Pa to 100Pa for 5 hours to obtain the banana oligosaccharide product;
[0045] (2) The remaining pomace after extraction of banana sugar liquid is used for enzymatic hydrolysis to prepare banana soluble and insoluble dietary fiber:
[0046] Step A: Add amylase to the pomace, the amount of amylase is 4.0% of the pomace weight, the action temperature is 95°C, add calcium chloride to make the calcium ion concentration 20mmol / L, adjust by adding hydrochloric acid and sodium hydroxide solution The p...
Embodiment 3
[0051] (1) Peel the bananas; beat the pulp for 2 minutes, extract with water, the weight-to-volume ratio of the material to liquid is 1g: 1ml, the temperature is 55°C, the extraction time is 55min, and the extraction is 5 times; the 5 extractions are combined, and the pomace is placed separately. The extract was filtered and concentrated in vacuum, precipitated with 4 times the volume of the extract in absolute ethanol, centrifuged at 4000 rpm for 13 minutes, collected the precipitate, and vacuum freeze-dried at 100Pa to 150Pa for 4 hours to obtain the banana oligosaccharide product;
[0052] (2) The remaining pomace after extraction of banana sugar liquid is used for enzymatic hydrolysis to prepare banana soluble and insoluble dietary fiber:
[0053] Step A: Add amylase to the pomace, the amount of amylase is 3.8% of the pomace weight, the action temperature is 98°C, add calcium chloride to make the calcium ion concentration 18mmol / L, adjust by adding hydrochloric acid and sod...
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