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Method for preparing functional microbial preparation from lactic acid bacillus and yeast

A functional microorganism, lactobacillus technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of arable land that can only be abandoned or planted with other crops, endogenous infection, poor clinical effect, etc. To achieve the effect of improving non-specific immunity, improving digestion and absorption capacity, and improving digestion and absorption function

Inactive Publication Date: 2010-06-02
SHANGHAI ENCHUANG BIOTECH RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In China, sulfonamides, tetracyclines, penicillins, chloramphenicol, kanamycin, gentamicin and other drugs have developed serious drug resistance in livestock and poultry, and the clinical effect is getting worse and worse, and the dosage is increasing accordingly. ; ③Because nitrogen, phosphorus, copper, zinc and pharmaceutical additives are discharged from the body of farmed animals, they cause pollution to the environment, resulting in deterioration of soil and groundwater quality, and release of various odors, etc.
According to experts' prediction, a pig farm with 10,000 heads is calculated according to the dosage of arsenic preparations allowed by the US FDA. If the arsenic-containing medicated feed is used continuously, nearly 1 ton of arsenic may be discharged to the surrounding pig farms after 5 to 8 years. After 16 years, the soil The content of arsenic in the sweet potato will increase by 0.28mg / kg. According to this calculation, within 10 years, the content of arsenic in the sweet potato produced in this area will all exceed the national food hygiene standard, and this piece of cultivated land can only be abandoned or planted with other crops; Decreased immunity, causing endogenous infection

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The first step: the source and cultivation of fermentation strains

[0039] a. Source of strains: Plant Lactobacillus and yeast of the present invention are all sourced from the Microorganism Culture Collection Center of the Ministry of Agriculture, wherein Lactobacillus plantarum (Lactobacillus plantarum), strain number: ACCC10533, beer yeast (Saccharomyces cerevisiae), strain number : ACCC20065;

[0040] b. Inoculate the isolated Lactobacillus plantarum and Saccharomyces cerevisiae into the broth medium respectively, and after culturing at 37°C for 22 hours, inoculate Saccharomyces cerevisiae on the yeast extract malt juice agar medium, and inoculate the Lactobacillus plantarum In the lactic acid bacteria culture medium, carry out streak culture, and select Lactobacillus plantarum and Saccharomyces cerevisiae with fast growth and obvious bacterial characteristics and store them in the laboratory;

[0041] c. Production and seed production: Finally, transfer the fast-...

Embodiment 2

[0064] The first step: the source and cultivation of fermentation strains: the same as in Example 1;

[0065] Step Two: Liquid Culture of Beneficial Bacteria

[0066] a. Weigh each strain according to the following weight ratio: 60% of Lactobacillus plantarum, 40% of Saccharomyces cerevisiae

[0067] b. Mix the weighed Lactobacillus plantarum and Saccharomyces cerevisiae together for compound culture: the mixed lawn weighed in step a is inoculated in a medium sterilized at 121° C. for 45 minutes at a ratio of 1:30. Stir in the fermenter for 30min at a speed of 200r / min. After stirring evenly, put it into a sterilized plastic bucket and let it stand for 7 days. During the standstill, measure the pH value every day and release the produced gas;

[0068] c. Sampling 3 times during the fermentation process, detecting the growth of the bacteria and measuring the pH value under a microscope, and stopping the fermentation when the total number of viable bacteria reaches 2 billion / ml...

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PUM

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Abstract

The invention relates to a method for preparing a functional microbial preparation from lactic acid bacillus and yeast, which is characterized in that: liquid of beneficial strains is adopted for culture; cell walls of the strains are collected centrifugally; a strain group is subjected to solid amplification culture; and a culture is added with the collected cell walls rich in polysaccharide. The method has the advantages that: high-concentration lactobacillus in the product can obviously improve the digestion and absorption of bred animals, and the generated metabolic products such as peptidogly can improve the immunity of the animals to pathogen; the high-concentration yeast in the product can improve rich single cell proteins required by the growth of the animals, and simultaneously the yeast cells are subjected to full wall breaking, and release a great amount of beta-1,3-glucan so as to obviously improve the immunity to Gram positive bacteria and Gram negative bacteria; and grain crops and common feeds are adopted as main raw materials for a culture medium, the production cost is reduced, and the method is easy to promote in animal breeding.

Description

technical field [0001] The invention relates to a preparation method for producing a functional microbial preparation by using lactobacillus and yeast. The preparation can be used to promote the growth of farmed animals and improve the immunity of the farmed animals, and belongs to the technical field of agricultural biological products. Background technique [0002] Over the years, due to the rapid expansion of the low cost of my country's animal breeding industry, the breeding environment has become out of control, animal immunity has declined, and the incidence of diseases has continued to rise. [0003] Since the discovery of antibiotics by Fleming in England in 1929, they have been widely used in the aquaculture industry, greatly improving the production level of the aquaculture industry. However, due to people's excessive pursuit of the economic benefits of breeding, the dosage of various feed additives such as antibiotics, hormones, and chemically synthesized drugs ha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/16C12N1/20C12N1/18C12R1/865C12R1/25A23K10/18
Inventor 江瀚
Owner SHANGHAI ENCHUANG BIOTECH RES INST
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