Human IgG and rheumatoid factor absorption device and related detection method of IgM antibody in human serum

Abstract

The invention provides a human IgG and a rheumatoid factor absorption device comprising a microplate which comprises at least one micropore, substances for specifically absorbing human IgG and rheumatoid factors are coated in the micropore; preferably, the microplate is prepared from polystyrene or polypropylene, and the number of the micropores ranges from 1 to 1536; the microplate also comprises a base, and can be arranged on the base in a dismountable manner; and the substances are goat anti-human IgG antibody and denatured / agglutinative human IgG. The invention also provides a method using the above device to detect IgM antibody in human serum. The human IgG and rheumatoid factor absorption device can specifically absorb human IgG and rheumatoid factors, thereby conveniently and rapidly removing the interference of human IgG and rheumatoid factors, being beneficial to the rapid and accurate realization of related detection, being applicable to large scale promotion and application, and providing guarantee for conveniently and rapidly performing large-scale clinical detection.

Description

technical field [0001] The present invention relates to the technical field of immunology, more specifically, to the technical field of devices for improving the accuracy of immunological detection, in particular to an adsorption device for human IgG and rheumatoid factor and a related method for detecting IgM antibody in human serum. Background technique [0002] The detection of IgM antibodies is interfered to varying degrees by specific IgG and rheumatoid factor (RF). Especially in the detection of IgM antibodies in the TORCH series (toxoplasma gondii, rubella virus, cytomegalovirus, herpes simplex virus, etc.) during pregnancy, due to the presence of more IgG and rheumatoid factors, the detection of IgM is more likely to be interfered. [0003] The main reason for the inaccurate detection of IgM ELISA (indirect method) is: there are specific IgG antibodies and IgM type RF in the serum, and the specific reasons are as follows: [0004] ①In the serum of patients with prev...

AI Technical Summary

Problems solved by technology

[0004] ①In the serum of patients with previous infection and chronic patients, the competitive adsorption of specific IgG antibody and specific IgM in the sample to be tested caused false negatives;

Ordinary IgG is also used as an adsorbent; however, the ability to adsorb RF is poor

In the existing Torch-IgM adsorption method, the sample (serum to be tested) is diluted and the main components of IgG / RF adsorption are integrated in a liquid, and the adsorption reaction and detection reaction are also completed in one hole, so Poor clearance of IgG and RF