Wheat bran polysaccharide with antitumor and immune regulation activities and extraction method thereof
A technology of wheat bran and polysaccharide, which is applied in the directions of antitumor drugs, organic active ingredients, medical preparations containing active ingredients, etc., can solve problems such as few related reports, and achieves low price, broad prospects for industrial development, and sources of rich effects
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Embodiment 1
[0035] Example 1, Extraction of Wheat Bran Immunomodulation and Antitumor Activity Polysaccharide
[0036] 1. Polysaccharide extraction: the flow chart of polysaccharide extraction is as follows figure 1 shown.
[0037] 1) Pretreatment of wheat bran
[0038] Weigh 100 g of wheat bran, disperse in 500 mL of dilute hydrochloric acid solution with a pH value of 3.5, and soak at 25°C for 12 hours. Centrifuge (1500rpm, 30min) to collect the precipitate; further wash the precipitate with water until the solution is clear, pass through a 200-mesh sieve, drain the water, and obtain de-phytic acid and de-starch wheat bran.
[0039] 2) Enzymolysis
[0040] Disperse the wheat bran de-phytic acid and de-starch into 400mL clear water, adjust the pH value of the system to 5.5, add 0.25g xylanase (the proportion of xylanase and wheat bran is 6.25U: 1g without pretreated wheat bran), placed in a 55°C insulation tank, stirred and extracted; after 4.0 hours of reaction, the enzymolyzed prod...
Embodiment 2
[0072] Example 2, Extraction of Wheat Bran Immunomodulation and Antitumor Activity Polysaccharide
[0073] 1. Polysaccharide extraction
[0074] 1) Pretreatment of wheat bran
[0075] Weigh 1 kg of wheat bran, disperse it in 4 L of dilute hydrochloric acid solution with a pH value of 3.0, and soak overnight (10 hours) at room temperature (23° C.). Centrifuge (1500rpm, 30min) to collect the precipitate; further wash the precipitate with water until the solution is clear, pass through a 200-mesh sieve, drain the water, and obtain de-phytic acid and de-starch wheat bran.
[0076] 2) Enzymolysis
[0077] Disperse the wheat bran of dephytic acid and destarch into 6L of clear water, adjust the pH value of the system to 5.0, add 4.5g xylanase (the ratio of xylanase and wheat bran is 8U: 1g wheat bran ), placed in a 60°C insulation tank, stirred and extracted; after reacting for 3.0 hours, the enzymolyzed product was obtained; the enzymolyzed product was heated to 95°C, kept for 10...
Embodiment 3
[0097] Example 3, Wheat Bran Immunomodulation and Extraction of Antitumor Active Polysaccharides
[0098] 1. Polysaccharide extraction
[0099] 1) Pretreatment of wheat bran
[0100] Weigh 5 kg of wheat bran, disperse in 20 L of dilute hydrochloric acid solution with a pH value of 3.0, and soak overnight (14 hours) at room temperature (27° C.). Centrifuge (1500rpm, 30min) to collect the precipitate; further wash the precipitate with water until the solution is clear, pass through a 200-mesh sieve, drain the water, and obtain de-phytic acid and de-starch wheat bran.
[0101] 2) Enzymolysis
[0102] Disperse phytic acid and destarched wheat bran into 35L of clear water, adjust the pH of the system to 5.3, add 20g of xylanase (the ratio of xylanase to wheat bran is 10U: 1g of wheat bran) , placed in a 50°C insulation tank, stirred and extracted; after 2.0 hours of reaction, the enzymolyzed product was obtained; the enzymolyzed product was heated to 95°C, kept for 10 minutes to...
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