Wheat bran polysaccharide with antitumor and immune regulation activities and extraction method thereof

A technology of wheat bran and polysaccharide, which is applied in the directions of antitumor drugs, organic active ingredients, medical preparations containing active ingredients, etc., can solve problems such as few related reports, and achieves low price, broad prospects for industrial development, and sources of rich effects

Active Publication Date: 2010-05-12
INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Research and product development in this area are carried out with wheat bran as raw material, and there are few related reports

Method used

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  • Wheat bran polysaccharide with antitumor and immune regulation activities and extraction method thereof
  • Wheat bran polysaccharide with antitumor and immune regulation activities and extraction method thereof
  • Wheat bran polysaccharide with antitumor and immune regulation activities and extraction method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1, Extraction of Wheat Bran Immunomodulation and Antitumor Activity Polysaccharide

[0036] 1. Polysaccharide extraction: the flow chart of polysaccharide extraction is as follows figure 1 shown.

[0037] 1) Pretreatment of wheat bran

[0038] Weigh 100 g of wheat bran, disperse in 500 mL of dilute hydrochloric acid solution with a pH value of 3.5, and soak at 25°C for 12 hours. Centrifuge (1500rpm, 30min) to collect the precipitate; further wash the precipitate with water until the solution is clear, pass through a 200-mesh sieve, drain the water, and obtain de-phytic acid and de-starch wheat bran.

[0039] 2) Enzymolysis

[0040] Disperse the wheat bran de-phytic acid and de-starch into 400mL clear water, adjust the pH value of the system to 5.5, add 0.25g xylanase (the proportion of xylanase and wheat bran is 6.25U: 1g without pretreated wheat bran), placed in a 55°C insulation tank, stirred and extracted; after 4.0 hours of reaction, the enzymolyzed prod...

Embodiment 2

[0072] Example 2, Extraction of Wheat Bran Immunomodulation and Antitumor Activity Polysaccharide

[0073] 1. Polysaccharide extraction

[0074] 1) Pretreatment of wheat bran

[0075] Weigh 1 kg of wheat bran, disperse it in 4 L of dilute hydrochloric acid solution with a pH value of 3.0, and soak overnight (10 hours) at room temperature (23° C.). Centrifuge (1500rpm, 30min) to collect the precipitate; further wash the precipitate with water until the solution is clear, pass through a 200-mesh sieve, drain the water, and obtain de-phytic acid and de-starch wheat bran.

[0076] 2) Enzymolysis

[0077] Disperse the wheat bran of dephytic acid and destarch into 6L of clear water, adjust the pH value of the system to 5.0, add 4.5g xylanase (the ratio of xylanase and wheat bran is 8U: 1g wheat bran ), placed in a 60°C insulation tank, stirred and extracted; after reacting for 3.0 hours, the enzymolyzed product was obtained; the enzymolyzed product was heated to 95°C, kept for 10...

Embodiment 3

[0097] Example 3, Wheat Bran Immunomodulation and Extraction of Antitumor Active Polysaccharides

[0098] 1. Polysaccharide extraction

[0099] 1) Pretreatment of wheat bran

[0100] Weigh 5 kg of wheat bran, disperse in 20 L of dilute hydrochloric acid solution with a pH value of 3.0, and soak overnight (14 hours) at room temperature (27° C.). Centrifuge (1500rpm, 30min) to collect the precipitate; further wash the precipitate with water until the solution is clear, pass through a 200-mesh sieve, drain the water, and obtain de-phytic acid and de-starch wheat bran.

[0101] 2) Enzymolysis

[0102] Disperse phytic acid and destarched wheat bran into 35L of clear water, adjust the pH of the system to 5.3, add 20g of xylanase (the ratio of xylanase to wheat bran is 10U: 1g of wheat bran) , placed in a 50°C insulation tank, stirred and extracted; after 2.0 hours of reaction, the enzymolyzed product was obtained; the enzymolyzed product was heated to 95°C, kept for 10 minutes to...

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Abstract

The invention discloses a wheat bran polysaccharide with antitumor and immune regulation activities and an extraction method thereof. The method for extracting the polysaccharide comprises the following step: performing enzymolysis of wheat bran with xylanase to obtain the polysaccharide, wherein the proportion of xylanase and wheat bran is (5U-12.5U):1g. The method of the invention adopts wheat bran which is a subsidiary agricultural product resource, to largely prepare the polysaccharide product by processing and extracting, the polysaccharide product mainly contains araboxylan (or pentosan); animal experiments show that the polysaccharide has the similar immune regulation and antitumor effects of the antitumor adjuvant drugs which are used in clinic; in addition, the price of raw materials of the polysaccharide is cheap and the sources are wide so that the polysaccharide of the invention has more market competition advantage and wide industrialized development prospect.

Description

technical field [0001] The invention relates to a wheat bran polysaccharide with antitumor and immune regulation activities and an extraction method thereof. Background technique [0002] Wheat bran is a by-product of wheat processing, mainly composed of wheat seed coat, aleurone layer and residual endosperm. Wheat bran is a typical grain dietary fiber raw material. Its composition is complex. In addition to the main cell wall constituent arabinoxylan (or pentosan), there are also cellulose, lignin, dextran, phytic acid, and starch. Wait. At present, in our country, most of wheat bran is used as feed for livestock and poultry and microbial fermentation medium. The high added value and in-depth research and development mainly focus on using it as a dietary fiber raw material to exert physiological functions such as laxative and blood fat reduction. , or as a dough improver or as an application function of edible glue. [0003] In domestic patents, wheat bran is mostly used...

Claims

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Application Information

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IPC IPC(8): C12P19/14C08B37/14A61K31/715A61P37/04A61P35/00A61K36/899
Inventor 周素梅曹丽王强刘红芝张晓娜任霞
Owner INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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