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Method for preparing radix actinidia chinensis polysaccharide extract

A technology for extracting kiwifruit root and polysaccharide is applied in the field of preparation of kiwifruit root polysaccharide extract, which can solve the problems of low extraction rate, purity and pharmacodynamic activity of kiwifruit root polysaccharide, increase hidden dangers in safety, complicated operation and the like, and improve production safety. , the effect of saving the amount of ethanol and reducing the amount of ethanol

Active Publication Date: 2010-05-12
ZHEJIANG CHINESE MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its disadvantages are low extraction rate, purity and medicinal activity of kiwifruit root polysaccharide, high energy consumption, cumbersome operation, long time consumption, high cost, and increased safety hazards due to the use of ether

Method used

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  • Method for preparing radix actinidia chinensis polysaccharide extract
  • Method for preparing radix actinidia chinensis polysaccharide extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Determination of the Active Part of the Kiwi Root Crude Polysaccharide Extract

[0030] Take 5000g of dried kiwi root decoction pieces, add 10 times the quality of water, heat and reflux extraction 3 times, 2 hours each time, combine the extracts, high-speed centrifugation (15000r / min, 20min), and pass the supernatant through a microporous membrane with a pore size of 0.45μm , the filtrate adopts ultrafiltration membranes with a molecular weight cut-off of 3 million, 1 million, 100,000, 30,000, and 5,000 in sequence, and performs ultrafiltration classification to obtain A (below 5,000), B (5,000-30,000), and C (30,000 -100,000), D (100,000-1 million), E (1 million-3 million), F (more than 3 million) and other 6 parts. Each part is concentrated and dried respectively, and its polysaccharide content is measured respectively, and its percentage in the total extract (polysaccharide) is calculated.

[0031] The extracts of kiwifruit roots from various parts were ...

Embodiment 2

[0035]Embodiment 2: the comparison of technology of the present invention and existing technology

[0036] Get the same batch of kiwifruit roots, extract the kiwifruit root polysaccharides respectively by methods such as literature process, patented process and process of the present invention, and compare (5 batches of each method):

[0037] Literature process (i.e. method 1 in the background technology part): dry 1000g of kiwifruit root decoction pieces, add 20 times the quality of water to extract, extract twice for 0.5h each time, add ethanol to the water extract to make the alcohol content reach 80% (v / v), standing overnight, suction filtration, to obtain the product.

[0038] Patented process (i.e. the method 2 of the background technology part): 1000g of dried kiwifruit root decoction pieces, add 5 times the mass of 30% (v / v) ethanol and soak twice, each time for 24 hours, let stand, take the supernatant, combine, Add ethanol to adjust the ethanol content to 72% (v / v)...

Embodiment 3

[0047] Embodiment 3: the preparation of kiwifruit root polysaccharide

[0048] Take 500 g of dried kiwi root decoction pieces, add 6 times the mass of water, heat and reflux for 1 hour, extract 3 times, combine the extracts, centrifuge at high speed (10000r / min, 20min), and pass the supernatant through a microporous membrane with a pore size of 0.65 μm. The filtrate is ultrafiltered with a membrane with a molecular weight cut-off of 3000000Da, and the ultrafiltrate is ultrafiltered with a membrane with a molecular weight cut-off of 5000Da, concentrated to 500ml, and the concentrated solution is added with ethanol to adjust the alcohol concentration to 55% (v / v), and stand for 60 hours at high speed Centrifuge (10000r / min, 20min), wash the precipitate once with absolute ethanol, and dry to obtain the crude polysaccharide from kiwifruit root. After measurement and calculation, the yield of crude polysaccharide from kiwifruit root is 2.07%, and the purity is 55.81%.

[0049] Take...

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Abstract

The invention provides a method for preparing radix actinidia chinensis polysaccharide extract. The method comprises: heating dried radix actinidia chinensis with water for reflux extraction; centrifuging extract liquid; passing supernatant through a microporous filter membrane; ultra-filtering filtrate; concentrating the obtained product till the content of crude medicinal material is 0.1 to 5 g / mL; adding concentrated solution to 85 to 95 percent ethanol till the volume concentration of ethanol is 50 to 90 percent; standing the obtained product for alcohol precipitation; performing centrifugation; washing precipitate; drying the washed precipitate under vacuum at 60 to 80 DEG C; obtaining crude radix actinidia chinensis polysaccharide; and obtaining refined radix actinidia chinensis polysaccharide through further purification. The method has the main advantages that: (1) the method adopted for preparing radix actinidia chinensis polysaccharide achieves maximum separation and purification effects, ensures high purity of radix actinidia chinensis polysaccharide and high extraction rate, enriches effective components, and improves efficacy; (2) the method greatly reduces ethanol consumption and lowers cost; and (3) the method greatly reduces ethanol consumption and does not need to use ether, thereby improving production safety.

Description

(1) Technical field [0001] The invention relates to a preparation method of kiwifruit root polysaccharide extract (including kiwifruit root crude polysaccharide and kiwifruit root refined polysaccharide). (2) Background technology [0002] Actinidia Chinensis (Radix Actinidia Chinensis), the root of Chinese Actinidia (Actindiachinensis Planch), also known as vine pear root, is recorded in "Chinese Pharmacopoeia" (1977 edition), and has the effects of clearing away heat, diuresis, promoting blood circulation, and reducing swelling. It is mainly used to treat gastrointestinal tumors and breast cancer, boils, scrofula, tuberculosis, hepatitis, etc. [0003] Modern pharmacological research has found that the polysaccharide component in kiwifruit root - Actindia Chinensis Planch Polysaccharide (ACPS) has the functions of anti-tumor, enhancing immune function, eliminating active oxygen free radicals, etc., and has no side effects. In recent years, pharmacological studies on kiwif...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 石森林李昌煜葛卫红吴瑾瑾夏聪华杨悦王丽丽
Owner ZHEJIANG CHINESE MEDICAL UNIVERSITY
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