Special culture medium for culturing yew plant cells to produce taxol

Abstract

The invention discloses a special culture medium for culturing yew plant cells to produce taxol, which is obtained by taking B5 culture medium as a basic formula and utilizing an orthogonal optimization test. When the basic component comparison production is carried out on the special culture medium and the B5 culture medium, the content of the taxol in the special culture medium is 4.2 times that of the B5 culture medium; when high-density of yew plant cells are planted, a combined test is carried out by adding silver nitrate, salicylic acid, chitosan, methyl jasmonic acid, tribasic ammonium citrate, lanthanum sulfate, phenylalanine, glycine, tryptophan and lysine in the culturing process, which can further improve the content of the taxol and shorten the production period of cells; and when the cells are cultured at the thirty-first day, the highest content of the taxol in the special culture medium is 285.15 mg / L which is 81 times the highest content of the taxol in the B5 culture medium, thus the taxol can be effectively produced by using the special culture medium for culturing yew plant cells to produce taxol.

Description

technical field [0001] The invention belongs to the field of biotechnology or biopharmaceuticals, and relates to a special medium for cultivating yew plant cells to produce paclitaxel. Background technique [0002] The medium commonly used to cultivate Taxus plant cells to produce paclitaxel is B 5 culture medium, but scientists such as Mei Xingguo found through experiments that in B 5 When the yew plant cells are cultured in the culture medium, it is easy to turn red and brown, resulting in a low content of paclitaxel produced by the cultured yew plant cells. Therefore, a two-step culture method is proposed to cultivate the yew plant cells to produce paclitaxel. Rapid growth in the culture medium to obtain a certain biomass of Taxus plant cells, and then transfer to the production medium for paclitaxel production to obtain cell cultures with high paclitaxel content, because B 5 The composition of the medium is complex, and a special production medium for cultivating taxus...

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Problems solved by technology

[0002] The medium commonly used to cultivate Taxus plant cells to produce paclitaxel is B 5 culture medium, but scientists such as Mei Xingguo found through experiments that in B 5 When cultivating yew plant cells in the culture medium, it is easy to turn red and brown, resulting in a low content of paclitaxel produced by cultivating yew plant cells. Therefore, a two-step culture method is proposed to cultivate yew plant cells to produce paclitaxel. Rapid growth in the medium to obtain a certain biomass of Taxus plant cells, and then transfer to the production medium for paclitaxel production to obtain a cell culture containing high paclitaxel, because B 5 The composition of the medium is complex, and a special production medium for cultivating taxus plant cells to promote the synthesis of paclitaxel has not been designed or optimized. The method of two-step culture to produce paclitaxel is generally selected B 5 As a production medium, the culture medium still has not solved the problem of low content when cultivating Taxus plant cells to produce paclitaxel

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