Method for quickly screening and testing cold resistance of tomatoes

Abstract

The invention discloses a method for quickly screening and testing the cold resistance of tomatoes, which comprises: firstly, culturing tomato seedlings growing for 3 to 5 weeks, and processing stem apex leaves at 3 to 5 DEG C for 1 to 3 hours and at 3 DEG C below zero to 5 DEG C below zero for 7 to 9 hours; secondly, restoring the stem apex leaves at 3 to 5 DEG C for 10 to 14 hours and placing the stem apex leaves in light at 20 to 22 DEG C for 14 to 18 hours; and finally, measuring the electrical conductivity of the cold-treated and restored leaves, placing the leaves in distilled water, performing vacuumization and volume fixing, shaking the leaves at 21 to 23 DEG C under a sealed condition for 1 to 3 hours, measuring an electrical conductivity C1, sealing the leaves, boiling the leaves for 10 to 20 minutes, cooking the leaves to room temperature, measuring an electrical conductivity C2 and calculating a relative conductivity according to a formula of C=(C1-C0) / (C2-C0)x100, whereinwhen the value domain of the relative conductivity C is less than or equal to 42 percent, the tomato variety is a cold-resistance variety. In the invention, the cold resistance screening of the wholesystem is performed under controllable conditions, the result is reliable, the method is simple, convenient and easy to master, the breeding period is shortened greatly and the method can be used fortest the cold resistance of an introduced variety at the same time.

Description

1. Technical field [0001] The invention relates to a method for identifying cold-resistant varieties of fruit and vegetable crops, in particular to a method for quickly screening and identifying cold-resistant varieties of tomatoes. 2. Background technology [0002] Low temperature freezing damage is a serious natural disaster in agricultural and forestry production. According to statistics, the world's annual losses are as high as 200 billion U.S. dollars. Therefore, the selection and breeding of cold-resistant crop varieties has become a key problem to be solved urgently in agricultural production. [0003] Tomato is a vegetable crop with relatively high economic benefits. Its fruit is rich in nutrition and contains multiple vitamins. It is one of the best nutritional foods for modern people. At the same time, tomato is also a health food, functional food and beauty cosmetics favored by ladies who love beauty. Tomatoes are originally produced in South America and are wid...

AI Technical Summary

Problems solved by technology

[0005] The present invention aims at the defects of existing screening and identification methods for tomato cold-resistant varieties, and aims to provide a fast, simple and effective screening and identification method to speed up the process of tomato cold-resistant breeding. The technical problem to be solved is the reliability of the screening and identification method, while avoiding the loss of germplasm

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