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Microbial preparation of bacillus cereus and method for treating nitrogen-containing wastewater by the preparation

A technology of Bacillus cereus and microbial preparation, applied in the field of microorganisms, can solve the problems of difficult transportation, complicated production process of bacterial preparation and high production cost

Inactive Publication Date: 2012-02-08
BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can solve the problem that different nitrification and denitrification bacteria groups need to be used for denitrification in traditional wastewater treatment, and there is no need for denitrification in anoxic environment and nitrification in aerobic environment. Difficult transportation, complex production process of bacterial agents, high production cost and many other problems

Method used

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  • Microbial preparation of bacillus cereus and method for treating nitrogen-containing wastewater by the preparation
  • Microbial preparation of bacillus cereus and method for treating nitrogen-containing wastewater by the preparation
  • Microbial preparation of bacillus cereus and method for treating nitrogen-containing wastewater by the preparation

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Experimental program
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Effect test

Embodiment 1

[0036] The screening of embodiment 1 bacillus cereus (Bacillus cereus) bacterial strain

[0037] This bacterial strain comes from our laboratory, using nitrate as a substrate, SBR aeration reactor A (total inorganic nitrogen (TIN) removal rate 60.0-83.0%, COD removal rate 85.0-90.0%) under high dissolved oxygen conditions domestication Activated sludge in , the preservation number is CGMCC NO.3047.

[0038] Utilizing the bromothymol blue contained in the BTB medium as an alkali production indicator, which turns blue when exposed to alkali, the mixed solution in the laboratory reactor A is evenly spread on the BTB culture by gradient dilution and coating method After several days of constant temperature and aerobic culture at 30°C, pick a single colony with a blue halo in the surrounding medium and different colony shapes as the primary screened aerobic denitrifying bacteria, and streak on the plate to purify the strain. The pure strain obtained by streak screening was stored ...

Embodiment 2

[0039] Embodiment 2: Bacillus cereus CGMCC NO.3047 aerobic denitrification culture method

[0040] In order to prevent the introduction of miscellaneous bacteria into the simulated wastewater, the nitrate simulated wastewater was inactivated in a high-temperature sterilization pot of 0.1MP for 20 minutes. Take 1g of the bacterial agent and inoculate it into a 250mL Erlenmeyer flask filled with 200mL of nitrate simulated wastewater to measure the denitrification and denitrification performance. Every 24 hours, use a needle filter equipped with a 0.2 μm mixed fiber membrane to filter and extract 5 mL of wastewater samples in the Erlenmeyer flask, and measure NO 2 - -N and NO 3 - -N concentration. By calculating NO 3 - -N reduction rate and total nitrogen (NH in wastewater 4 + -N, NO 3 - -N and NO 2 - -N concentration sum) removal rate analysis strain NO 3 - -N reduction performance and denitrification performance.

[0041] Nitrate simulated wastewater ( / L): sodium...

Embodiment 3

[0042] Embodiment 3: the assay method of the aerobic denitrification performance of bacillus cereus CGMCC NO.3047

[0043] The conditions for aerobic denitrification of wastewater are: the dissolved oxygen concentration is realized by controlling different rotational speeds rpm. The rotational speed is 100 rpm, and the corresponding dissolved oxygen concentration is 4.2-5.6 mg / L; the nitro nitrogen concentration is about 63.8 mg / L; the carbon-nitrogen ratio is 10 , pH value is 6.5.

[0044] Carry out denitrification performance measurement according to embodiment 2 method, test result: NO after 4 days 3 - The -N reduction rate was 78.0%, and the TIN removal rate was 76.8% after 4 days.

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Abstract

The present invention relates to Bacillus cereus (Bacillus cereus) microbial preparation and preparation method thereof, and described Bacillus cereus preservation number is CGMCC NO.3047, and its steps are as follows: Utilize agar and polyvinyl alcohol to prepare Bacillus cereus CGMCC No. 3047 was immobilized and dried by air flow at 20-60°C to finally make a biological agent. The use of agar and polyvinyl alcohol in the preparation method is small, the production cost is low, and the preparation method is simple; the biological bacterial agent prepared by the method has high microbial activity, is easy to store, is convenient to transport, and is easy to recycle; use this microbial agent to treat nitrogen-containing Wastewater, under the conditions of NH4+-N initial concentration at 100mg / L, NO3--N initial concentration at 30mg / L, the removal rate of NH4+-N reaches 93.7%, TN (NH4+-N, NO3--N and The removal rate of NO2--the sum of N concentrations) reaches 88.6%; another feature of this method is that the strain can simultaneously remove NH4+-N, NO3--N and NO2--N. Therefore, in view of the above characteristics, this method is not only suitable for various urban and domestic wastewater, but also suitable for the denitrification treatment of industrial wastewater with high concentration of ammonia nitrogen.

Description

technical field [0001] The invention relates to a strain of bacillus cereus with denitrification performance, a microbial preparation prepared by the strain and a method for treating nitrogen-containing wastewater by using the microbial preparation, belonging to the field of microorganisms. Background technique [0002] In the traditional wastewater denitrification method, ammonia nitrogen needs to be converted into nitro nitrogen by nitrifying bacteria, and then the nitro nitrogen in water is reduced to gaseous state by denitrifying bacteria, and then removed from the water. However, in recent years, many literature reports have found that some denitrifying bacteria also have nitrifying properties. This kind of bacteria can directly convert ammonia nitrogen into the gaseous form of nitrogen and discharge nitrogen directly from the water body. This bacterium has high utilization value, and can reduce denitrification steps in waste water denitrification, save time and improve...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/04C02F3/34C12R1/085
Inventor 汪苹王磊廖小红尹明锐刘健楠
Owner BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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