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Method of producing edible immobilized active probiotics

A technology of active probiotics and production methods, applied in the field of production of edible immobilized active probiotics, to achieve the effect of increasing the number of bacteria

Active Publication Date: 2010-04-07
哈尔滨美华生物技术股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, although probiotic products are extremely abundant on the market, there are only two forms of probiotic products: liquid probiotics and probiotic freeze-dried powder

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] (1) Culture the Lactobacillus casei test tube slant strain for 12-72 hours, then insert it into the liquid medium of the Erlenmeyer flask for culture, and cultivate it at 37°C for 20 hours, then carry out the expansion culture step by step, and then carry out the fermentation culture in the fermenter. Cultivate at 37°C for 20 hours, so that the total number of bacteria in the fermentation broth reaches 1.0×10 9 / mL, this culture solution is used as seed solution;

[0016] (2) Add 3.0% of the cultivated and mature seed liquid into the sterilized and cooled sodium alginate immobilized carrier solution (A liquid), and mix thoroughly to prepare (B liquid).

[0017] (3) Then, drop the immobilized carrier solution (solution B) into 4% calcium chloride (solution C) to solidify it. The solidified immobilized carrier containing Lactobacillus casei is washed with sterile water, and then placed in a closed culture at 10° C.-45° C. for 8-72 hours.

[0018] (4) Preparation of immo...

Embodiment 2

[0022] (1) Culture the Lactobacillus casei test tube slant strain for 12-72 hours, then insert it into the liquid medium of the Erlenmeyer flask for culture, and cultivate it at 37°C for 20 hours, then carry out the expansion culture step by step, and then carry out the fermentation culture in the fermenter. Cultivate at 37°C for 20 hours, so that the total number of bacteria in the fermentation broth reaches 1.0×10 9 / mL, this culture solution is used as seed solution;

[0023] (2) Add 2.0% of the cultivated and mature seed solution into the sterilized and cooled κ-carrageenan immobilized carrier solution (A solution), and mix thoroughly to prepare (B solution).

[0024] (3) Then, drop the immobilized carrier solution (solution B) into 4% potassium chloride (solution C) to solidify it. The solidified immobilized carrier containing Lactobacillus casei was washed with sterile water, and then placed at 37° C. for airtight culture for 20 hours.

[0025] (4) Preparation of immob...

Embodiment 3

[0029] (1) Cultivate Lactobacillus rhamnosus and Lactobacillus acidophilus on the slant of the test tube for 12-72 hours, then respectively insert them into the liquid culture medium of the Erlenmeyer flask, cultivate them at 37°C for 20 hours, and then carry out the expansion culture step by step, Then carry out fermenter fermentation culture again, cultivate 20 hours at 37 ℃, make the total number of bacteria in the fermented liquid reach 1.0×10 9 / mL, the culture solution is used as the seed solution, and the two solutions are mixed in equal volumes;

[0030] (2) Add 2.0% of the cultivated and mature seed liquid into the sterilized and cooled mixed solution of sodium alginate and κ-carrageenan immobilized carrier (liquid A), and mix thoroughly to prepare (liquid B).

[0031](3) Then, drop the immobilized carrier solution (B solution) into the mixed solution of 4% calcium chloride and 4% potassium chloride (C solution) to make it solidify. The solidified immobilized carrier...

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PUM

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Abstract

The invention discloses a method of producing edible immobilized active probiotics, comprising the following steps: adding one kind of or various kinds of mature probiotics strains to immobilized carrier solution which is suitable for the growth and the propagation of the probiotics strains according to a certain proportion; adding dropwise the immobilized carrier solution which comprises one kind of or various kinds of probiotics strains into immobilized solution, or adding the immobilized solution to immobilized carrier to solidify the immobilized carrier. The solidified immobilized carrier which comprises one kind of or various kinds of probiotics strains is washed by sterile water and then is sealed to be cultured for 8-72 hours at 10-45 DEG C. The produced immobilized probiotics do not need to be separated and frozen to dry, can be taken directly as probiotics and can be put in other food to take. If being sealed to store at normal temperature for a long time, the immobilized probiotics can grow without stop.

Description

(1) Technical field [0001] The invention relates to a production method of active probiotics, in particular to a production method of edible immobilized active probiotics. (2) Background technology [0002] Probiotics are a kind of beneficial microorganisms that improve the micro-ecological balance of the human intestinal tract and have a conditioning effect on human health. They mainly promote human health by maintaining the micro-ecological balance of the intestinal tract. Its physiology The function is generally recognized by the scientific and technological circles all over the world. There are a lot of papers published every year on the study of probiotics all over the world. In summary, probiotics have the following functions: [0003] (1) Improving immunity: Bifidobacterium and Lactobacillus acidophilus can produce a large amount of immunoglobulin. Thereby activating the body's immune cells, making it difficult for exogenous bacteria to remain in the body, and achie...

Claims

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Application Information

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IPC IPC(8): C12N11/00A23L1/29A61P1/10A61P1/14A61P3/02A61P3/06A61P17/16A61P35/00A61P37/04A61P39/06C12R1/01C12R1/23C12R1/46C12R1/225C12R1/245C12R1/25A23L33/00
Inventor 梁金钟林枫翔
Owner 哈尔滨美华生物技术股份有限公司
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