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Extracts of aster koraiensis, and pharmaceutical composition and functional food comprising the same

A technology of extract and composition, applied to Korean aster extract, can solve problems such as toxicity

Inactive Publication Date: 2010-03-24
KOREA INST OF ORIENTAL MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Aminoguanidine is the most promising candidate synthetic drug for the prevention and treatment of diabetic complications. It has entered the third phase of clinical trials, but there is a problem of toxicity caused by long-term use

Method used

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  • Extracts of aster koraiensis, and pharmaceutical composition and functional food comprising the same
  • Extracts of aster koraiensis, and pharmaceutical composition and functional food comprising the same
  • Extracts of aster koraiensis, and pharmaceutical composition and functional food comprising the same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0077] Example: Extraction and Fractionation of Korean Aster

[0078]In September 2004, leaves, stems, roots and flowers of Aster aster were collected in Muju-gun, Jeollabuk-do, Korea. The collected plants are classified according to leaves and stems, roots, flowers, and cut into thin slices respectively. Each chopped part was added to 80% ethanol, placed in an extractor, and extracted 3 times at room temperature, each time for 7 days. The extract was concentrated under reduced pressure, thus obtaining leaf-stem extract, root extract and flower extract. During the concentration process, the temperature of the extractor is maintained at 40-45°C in order to prevent decomposition and hydrolysis of the plant components. After complete removal of ethanol by vacuum concentration, the extract was placed in a freeze dryer to remove moisture and dry.

[0079] Such as figure 1 As shown, the leaf and stem extracts, root extracts and flower extracts of Aster aurantia were obtained a...

experiment example 1

[0080] Experimental example 1: Analysis of the inhibitory effect of the inventive material on advanced glycation end products

[0081] Bovine serum albumin (hereinafter referred to as BSA; Sigma, USA) was selected as an in vitro test to detect leaf and stem extracts, root extracts and flower extracts and various extract fractions on the production of advanced glycation end products. The protein source of the inhibitory effect. Dissolve BSA with 50 mM phosphate buffer (pH 7.4) to a final concentration of 10 mg / ml. 0.2M fructose and 0.2M glucose were used as sugar sources. Add the fructose / glucose mixture to the prepared BSA solution. The leaf and stem extracts, root extracts and flower extracts and the n-hexane fractions, ethyl acetate fractions, n-butanol fractions and water fractions of the extracts were dissolved in distilled water as test groups. Next, each solution was added to the BSA / sugar mixture, and cultured at 37°C for 14 days.

[0082] At this time, 0.02% sodi...

experiment example 2

[0093] Experimental example 2: Analysis of the inhibitory effect of the inventive extracts and fractions on the activity of aldose reductase

[0094] The leaf and stem extracts, root extracts and flower extracts of Aster korean, as well as various extracts n-hexane fractions, ethyl acetate fractions, n-butanol fractions and water fractions were dissolved for in vitro tests to analyze their effect on aldose reductase activity inhibition.

[0095] Detection method

[0096] According to the method of Dufrane (1984), in order to obtain natural aldose reductase from the eyeball of SD rat (Sprague-Dawley rat, 250-280g), the lens collected from the rat was in 135mM sodium, potassium phosphate buffer (pH 7.0) and 10mM 2-mercaptoethanol buffer solution with a homogenizer and ultrasonic breaker. The obtained solution was centrifuged at 14000 rpm for 30 minutes, and the supernatant was filtered through a 0.2-μm filter and used for the test. All the above processes were carried out a...

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Abstract

Disclosed herein are a leaf and stem extract of Aster koraiensis, a root extract of Aster koraiensis, a flower extract of Aster koraiensis, a pharmaceutical composition for the prevention or treatment of diabetic complications, a health functional food for the improvement of diabetic complications, and a pharmaceutical composition and health functional food for the prevention or delay of aging, in which each of the compositions and the foods contain, as an active ingredient, at least one of the Aster koraiensis extracts. Each of the extracts is obtained by drying and finely cutting each of the leaf and stem part, root and flower of Aster koraiensis, extracting the cut plant with alcohol or aqueous alcohol solution, filtering the extracted solution and concentrating the filtrate under reduced pressure. Also disclosed are a pharmaceutical composition for the prevention or treatment of diabetic complications, a health functional food for the improvement of diabetic complications, and a pharmaceutical composition and health functional food for the prevention or delay of aging, which contain, as an active ingredient, at least one of fractions obtained by fractionating each of the Aster koraiensis extracts in the order of hexane, ethyl acetate, n-butanol and water fractions. The leaf and stem extract of Aster koraiensis, the root extract of Aster koraiensis and the flower extract of Aster koraiensis have the effect of inhibiting the production of advanced glycation endproducts (AGEs), the causes of diabetic complications, and effectively inhibit aldose reductase (AR) activity. Thus, the disclosed extracts will be highly useful for the prevention or treatment of diabetic complications and the prevention of aging.

Description

technical field [0001] The present invention relates to a leaf and stem extract of Aster koraiensis, a root extract of Aster koraiensis, a flower extract of Aster koraiensis, and a drug combination for preventing or treating complications of diabetes Extracts and functional foods containing at least one of said extracts as active ingredients, especially leaf and stem extracts, root extracts and flower extracts, which not only have the ability to inhibit advanced glycation which is an important factor in inducing diabetic complications The production of the end product also has the function of inhibiting the activity of aldose reductase, thereby effectively preventing and treating diabetic complications, and used as a pharmaceutical composition and functional food for preventing or treating diabetic complications. Background technique [0002] Diabetes is one of the major adult diseases in the world, and recently, its incidence has reached 7 to 8% in Korea along with rapid ec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/28
CPCA23V2002/00A61K36/28A23L1/3002A23L33/105A61P3/10A23V2200/328A23V2250/21
Inventor 金真淑张大植李允美金种敏金英淑孙银珍金赞植金正玹金温顺郑东昊刘姃林金周焕
Owner KOREA INST OF ORIENTAL MEDICINE
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