Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Primers, probes and kits for detection of coxsackie virus a16 nucleic acid

A Coxsackie virus, A16 technology, applied in the field of probes, kits, and primers for Coxsackie virus A16 nucleic acid detection, can solve the problems of cumbersome steps, long detection period, easy to miss detection, etc., and achieve high accuracy , high sensitivity, good primer specificity

Inactive Publication Date: 2012-02-01
何雅青 +1
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The classic methods for detecting enteroviruses mostly use cell culture and specific antibody detection. This method has cumbersome steps and a long detection cycle, and some enteroviruses are difficult to proliferate in cells, and are prone to missed detection; traditional RT-PCR is also used Used to detect EV71 and CA16, but the detection time takes 6 hours

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Primers, probes and kits for detection of coxsackie virus a16 nucleic acid
  • Primers, probes and kits for detection of coxsackie virus a16 nucleic acid
  • Primers, probes and kits for detection of coxsackie virus a16 nucleic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0020] The following examples are used to further illustrate the present invention, but are not intended to limit the scope of the present invention.

[0021] Design and synthesis of primers and probes:

[0022] According to the CA16VP1 gene sequence in GenBank, primers and Taqman probes were designed at its conserved sites using the biological software Primer Express V2.0, and Blast homology comparison verification was carried out through the NCBI database. The primer sequences are:

[0023] CA16Pf (Forward): 5'-GAACCATCACTCCACACAGGAG-3'

[0024] CA16Pr (reverse): 5'-GTACCCGTGGTGGGCATTG-3'

[0025] The sequence of the probe is: 5'-CAGCCATTGGGAATTTCTTTAGCCGTG-3'

[0026] The 5' end of the probe was labeled with the reporter fluorescent dye VIC, and the 3' end was labeled with the quencher fluorescent dye BHQ2.

[0027] Extraction of RNA:

[0028] Extract RNA from virus samples with Roche High Pure viral RNA kit, take about 0.5 g of feces, add 500 μl of TE to make a 10...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to a primer, a probe and a kit for detecting Coxsackievirus A16 nucleic acid. The nucleotide sequence of the primer is: forward primer 5'-GAACCATCACTCCACACAGGAG-3'; reverse primer 5'-GTACCCGTGGTGGGCATTG-3'. The nucleotide of the probe is 5'-CAGCCATTGGGAATTTCTTTAGCCGTG-3'. Also provided is a method for detecting Coxsackievirus A16 nucleic acid. The method uses sample RNA as a template, uses the above primers and probes to perform real-time fluorescent RT-PCR amplification, and judges the result according to the amplification curve after each cycle. The invention has good primer specificity, fast and simple detection method, high accuracy and high sensitivity, and provides scientific basis for etiological diagnosis and differential diagnosis of hand, foot and mouth disease and related diseases.

Description

technical field [0001] The invention relates to biological detection technology, in particular to primers, probes and kits for coxsackievirus A16 nucleic acid detection. Background technique [0002] Coxsackievirus A16 (Coxsackievirus A16, CA16 for short) is a single-stranded positive-sense RNA virus belonging to the Picoranviridae family. CA16 is an important pathogen that causes hand, foot and mouth disease in children. The clinical symptoms caused by it are difficult to distinguish from hand, foot and mouth disease caused by enterovirus 71 infection. However, under normal circumstances, CA16 infection generally does not cause serious neurological related diseases. , is often overlooked due to its subclinical infection. Many countries and regions in the world have reported the prevalence of hand, foot and mouth disease caused by CA16, and there is a pandemic every 3-5 years. In recent years, CA16 has been paid more and more attention due to its association with myocardit...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/70C12Q1/68C12N15/11
Inventor 何雅青肖性龙
Owner 何雅青
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com