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Method for brewing a yellow wine using enzyme preparation and multi-bacteria

A technology of multi-strain and enzyme preparation, which is applied in the field of wine making, can solve the problems of increasing rice wine, poor saccharification and liquefaction effects, rancidity of mash, etc., and achieves the effects of improved wine yield, rich aroma and full body

Inactive Publication Date: 2012-07-25
SHANGHAI CHUANGBO ECOLOGICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The traditional rice wine process uses naturally cultivated wheat bran koji to ferment the base material, mainly using the enzymes and yeast produced in the bran koji. Although it has the advantages of low cost and simple process, there are few enzymes in the bran koji. Low activity, poor saccharification or liquefaction of the substrate, low utilization rate of the substrate, and no measures to inhibit the production of miscellaneous bacteria, so it is easy to be contaminated by bacteria, which will cause the mash to become rancid and deteriorate, and brewing failure
Although the current health-care rice wine process has been greatly improved and improved in the sterilization and koji making processes, it is mainly to add some biological enzyme preparations and add medicinal Chinese herbal medicines in the blending. These enzyme preparations are of a single type and are foreign-aided. Addition, its saccharification and liquefaction effects are poor, and the added Chinese herbal medicine components are complex, and the medicinal effects are somewhat antagonistic to each other, which cannot bring about a comprehensive effect
A Chinese invention patent has been published, titled: "Processing Method of Low-Nutrition Rice Wine", application number: 200510049970.1, publication number CN1710051A, its main feature is that a certain amount of stachyion is added in the blending process of the traditional rice wine production process Sugar, isomaltooligosaccharide, and bamboo leaf extract only increase the ingredients of rice wine from foreign aid, and cannot overcome the problems of easy bacterial contamination in the brewing process, easy antagonism of simple addition of nutrients, and product homogeneity.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A kind of method utilizing enzyme preparation and multi-bacteria brewing yellow rice wine is:

[0030] The first step: steaming and cooling of rice

[0031] Soak glutinous rice with 2 times the volume of clear water for 2 days, add 30,000 units / ml of lipase 0.07vol% and steam in the oven for 25 minutes, lower the temperature of the steamed raw materials to 60°C and keep it constant;

[0032] The second step: insulation liquefaction

[0033] Add 0.12vol% of α-amylase of 2-40,000 units / gram and 0.15vol% of neutral protease of 20,000-40,000 units / gram to the glutinous rice steamed and cooled in step 1, heat and liquefy for 2 hours to obtain mash;

[0034] The third step: insulation saccharification

[0035] Use lactic acid to adjust the pH of the mash obtained in step 2 to 4.5, use 50,000 units / ml of acid-resistant glucoamylase 0.2%, keep warm for saccharification for 40 minutes, and cool to room temperature;

[0036] The fourth step: drop tank main fermentation

[003...

Embodiment 2

[0045] A kind of method utilizing enzyme preparation and multi-bacteria brewing yellow rice wine is:

[0046] The first step: steaming and cooling of rice

[0047] Soak the glutinous rice in 2.5 times the volume of clear water 3, add 30,000 units / ml of lipase 0.07vol% and steam in the oven for 30 minutes, lower the temperature of the steamed raw materials to 63°C and keep it constant;

[0048] The second step: insulation liquefaction

[0049] Add 2-40,000 units / gram of a-amylase 0.14vol% and 2-40,000 units / gram of neutral protease 0.16vol% to the glutinous rice steamed and cooled in step 1, heat and liquefy for 2.5 hours to obtain mash;

[0050] The third step: insulation saccharification

[0051] With embodiment 1.

[0052] The fourth step: drop tank main fermentation

[0053]Add 0.23vol% bran koji, 0.25vol% rice wine yeast, 0.06vol% Torulopsis and 0.02vol% lactic acid bacteria to the mash obtained in step 3, and ferment at 29°C for 5-7 days while saccharifying until alco...

Embodiment 3

[0061] A kind of method utilizing enzyme preparation and multi-bacteria brewing yellow rice wine is:

[0062] The first step: steaming and cooling of rice

[0063] Soak glutinous rice with 2.5 times the volume of clear water for 3 days, add 30,000 units / ml of lipase 0.07vol% and steam in the oven for 30 minutes, and lower the temperature of the steamed raw materials to 65°C to keep it constant;

[0064] The second step: insulation liquefaction

[0065] Add 2-40,000 units / gram of a-amylase 0.15vol% and 2-40,000 units / gram of neutral protease 0.18vol% to the glutinous rice steamed and cooled in step 1, heat and liquefy for 3 hours to obtain mash;

[0066] The third step: insulation saccharification

[0067] Use lactic acid to adjust the pH of the mash obtained in step 2 to 5, use 50,000 units / ml of acid-resistant glucoamylase 0.3%, heat-preserve and saccharify for 40 minutes, and cool to room temperature;

[0068] The fourth step: drop tank main fermentation

[0069] Add 0.2...

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Abstract

The invention provides a method for brewing a yellow wine using enzyme preparation and multi-bacteria, characterized in that: the concrete steps are that: soaking glutinous rice in clean water and adding lipase; steaming the glutinous rice and cooling, and adding alpha-amylase and neutral protease into the mixture and incubating and liquefying the mixture; adjusting the pH of mash and adding acid-resistant glucoamylase and incubating, saccharifying and cooling the mixture; adding bran koji, saccharomyces cerivisiae, ester-producing yeast and saccharifying and fermenting the mixture until the alcohol content is 12 degrees; continuously fermenting the mixture until the alcohol content is larger than 15 degrees and squeezing and filtering the mixture; adding stachyose, oligoisomaltose, honey, medlar leach solution and lycopene antioxidant into the filtered solution; processing the above mixture using a microwave alcoholic ripening machine and adding diatomite absorbent into the mixture and quickly freezing the mixture to clarify the mixture and then using a diatomite filter machine to filter the mixture and finally using membrane micro-straining technology to strain and degerm the product. The advantages of the invention are that: the wine taste is rich and strong; the wine body is full and soft and the wine is a health-care wine capable of adjusting the micro-ecological balance of the human digestive system.

Description

technical field [0001] The technology of the invention relates to a method for brewing rice wine by using an enzyme preparation and multi-bacteria, and belongs to the technical field of wine making. Background technique [0002] The traditional rice wine process uses naturally cultivated wheat bran koji to ferment the base material, mainly using the enzymes and yeast produced in the bran koji. Although it has the advantages of low cost and simple process, there are few enzymes in the bran koji. The activity is low, the effect of saccharification or liquefaction of the substrate is poor, and the utilization rate of the substrate is low. At the same time, there are no measures to inhibit the production of miscellaneous bacteria, so it is easy to be contaminated with bacteria, which will cause the rancidity of the mash and the failure of brewing. Although the current health-care rice wine process has been greatly improved and improved in the sterilization and koji making proces...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12G3/02C12G3/06
Inventor 江瀚
Owner SHANGHAI CHUANGBO ECOLOGICAL ENG
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