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Reaction unit for sludge acclimation and polyhydroxyalkanoates synthesis by sludge and synthetic method thereof

A technology of polyhydroxyalkanoate and synthesis reaction, which is applied in the field of biodegradable material polyhydroxyalkanoate synthesis, can solve the problems that the yield and accumulation time of polyhydroxyalkanoate cannot meet the requirements of industrialization, and achieve structural The effect of simplicity, simple conditions, and convenient operation

Inactive Publication Date: 2012-08-22
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although the above-mentioned patents and documents have discussed and studied the process of synthesizing polyhydroxyalkanoate from sludge, the yield and accumulation time of synthesizing polyhydroxyalkanoate from sludge cannot meet the requirements of industrialization.

Method used

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  • Reaction unit for sludge acclimation and polyhydroxyalkanoates synthesis by sludge and synthetic method thereof
  • Reaction unit for sludge acclimation and polyhydroxyalkanoates synthesis by sludge and synthetic method thereof
  • Reaction unit for sludge acclimation and polyhydroxyalkanoates synthesis by sludge and synthetic method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0019] The reaction device for domesticating sludge and synthesizing polyhydroxyalkanoate from sludge in the present invention includes a sludge domesticating device and a synthesis reactor.

[0020] The sludge domestication device is provided with a domestication reaction tank 3 and a domestication reaction aeration head 4, and the domestication reaction aeration head 4 is placed at the bottom of the domestication reaction tank 3, and the air inlet of the domestication reaction aeration head 4 is connected to the device through a connecting pipe. It is connected to the rotameter 2 and the air compressor 1 outside the domestication reaction tank 3, the aeration rate of the domestication reaction aeration head 4 is controlled by the rotameter 2, the total volume of the domestication reaction tank 3 is 6L, and the domestication reaction aeration head 4. Sand core aeration heads are used, and a mud discharge port 31 with a valve is provided at every 1L of the tank wall of the dome...

Embodiment 2～7

[0025] The sludge synthesis of PHAs was carried out by changing different acetic acid dosages and different pH values. Other methods and conditions were the same as in Example 1. The results of sludge accumulation of PHAs are shown in Table 1.

[0026] Table 1 Comparison of main parameters of sludge accumulated PHAs under different carbon source dosage and pH value

[0027]

[0028] By embodiment 1 method, get 5L MLSS and be the activated sludge of 1g / L, add with CH 3 COOH 0.4mL / L, KCl117mg / L, K 2 HPO 4 ·3H 2 O 75mg / L, NH 4 Cl 97mg / L, MgSO 4 75mg / L, CaCl 2 27mg / L, FeCl 3 ·6H 2 05mg / L, peptone 167mg / L, and yeast extract 62mg / L, adjust the pH value to 7.0, and the aeration rate is 1L / L / min. After 19 hours of aeration, stop the aeration, and open the mud discharge port to discharge Sludge, control the sludge concentration in the acclimation reaction tank to 1g / L, discharge 3L supernatant after sedimentation for 20min, and a cycle is 20h; then "water intake - ae...

Embodiment 8

[0031] By embodiment 1 method, get 5L MLSS and be the domestication reaction tank that the activated sludge of 2g / L drops into the sludge domestication device, add with CH 3 COOH 0.75mL / L, KCl 234mg / L, K 2 HPO 4 ·3H 2 O 150mg / L, NH 4 Cl 194mg / L, MgSO 4 150mg / L, CaCl 2 54mg / L, FeCl 3 ·6H 2 O 10mg / L, peptone 334mg / L, yeast extract 124mg / L prepared medium, adjust the pH value to 9.0, control the aeration rate by rotameter to 2L / L / min, after aerobic aeration for 23h, stop Aerate, open the sludge outlet to discharge sludge, control the sludge concentration in the domestication reaction tank to 2g / L, discharge 3L supernatant after 30min of sedimentation, and a cycle is 24h; then "water intake - aerobic aeration - sludge discharge - After 10 cycles of "sedimentation-effluent" culture and domestication, the sludge properties were stable, the appearance gradually changed from black to light brown, and the sludge volume index (SVI) was 102mL / g.

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Abstract

The invention discloses a reaction unit for sludge acclimation and polyhydroxyalkanoates synthesis by sludge and a synthetic method thereof, and provides a reaction unit which is applicable for large-scale industrial production and has simple structure, convenient operation and low cost for sludge acclimation and polyhydroxyalkanoates synthesis by sludge as well as a method which adopts the reaction unit and has higher sludge synthetic yield for sludge acclimation and polyhydroxyalkanoates synthesis by sludge. The reaction unit is provided with a sludge domestication device and a synthesis reactor; the sludge domestication device is provided with a domestication retort and an aerator; and the synthesis reactor is provided with a constant temperature water bath, a synthesis retort, two microcomputer time controllers, an aerator and a stirrer. Activated sludge is taken and put into the domestication retort, continuous domestication is conducted by taking water feed, aerobic aeration, spoil disposal, sedimentation and water outlet as a domestication period to obtain stably-domesticated sludge, then the sludge is put into the synthesis retort while taking acetic acid as the carbon source and adjusting Ph, and PHAs is accumulated in a circular and intermittent aeration method of aeration, aeration stopping and stirring.

Description

technical field [0001] The invention relates to a method for synthesizing polyhydroxyalkanoate (PHAs), a biodegradable material, in particular to a method for biosynthesizing polyhydroxyalkanoate by using excess activated sludge. Background technique [0002] The use of microorganisms to produce degradable plastic polyhydroxyalkanoates (PHAs) has received extensive attention since the oil crisis in 1970 and the environmental protection movement. Due to their unique physical and chemical properties of biocompatibility and biodegradability, PHAs have broad application prospects in the fields of medicine, tissue engineering, plastic products, and piezoelectric products. At present, the research on the biosynthesis of PHAs has become a hot topic, and some countries have carried out the industrial production of bacterial fermentation PHAs. Although the research on pure bacteria fermentation methods is relatively sufficient at home and abroad, and the technology is relatively mat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/62C12M1/00
CPCC12M27/02C12M41/26
Inventor 李清彪刘正贵王远鹏何宁王海涛孙道华王婧
Owner XIAMEN UNIV
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