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Silica-coated fluorescent magnetic nanoparticle, preparation method and application

A technology of magnetic nanoparticles and silicon shells, applied in the preparation of the above-mentioned silicon shell fluorescent magnetic nanoparticles, the application of the above-mentioned silicon shell fluorescent magnetic nanoparticles, and the field of silicon shell fluorescent magnetic nanoparticles, can solve the toxic effect, toxic light instability issues of sex

Inactive Publication Date: 2010-03-17
CAPITAL UNIVERSITY OF MEDICAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the surface of nanoparticles is not modified in any way, organic fluorescent molecules such as FITC on the surface may cause potential toxic effects once they come into contact with cells or tissues
In addition, the organic dyes encapsulated inside the silica can also produce toxicity and photostability due to possible leakage

Method used

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  • Silica-coated fluorescent magnetic nanoparticle, preparation method and application
  • Silica-coated fluorescent magnetic nanoparticle, preparation method and application
  • Silica-coated fluorescent magnetic nanoparticle, preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Fe 3 o 4 Preparation of:

[0057] Synthesis of Fe coated with oleic acid by Lopez-Lopez et al method 3 o 4 Magnetic nanoparticles: 3.04g FeCl 3 ·6H 2 O with 2.65g (NH 4 ) 2 Fe(SO 4 ) 2 ·6H 2 O mixed (Fe 3+ : Fe 2+ =2:1.2), dissolved in 40ml of water, under vigorous stirring, quickly added 8ml of NH 3 ·H 2 O and 0.8ml oleic acid, N 2 React under protection for 1 hour, heat up to 95°C, cool down, adjust the pH to 5-6, and separate black Fe from the reaction solution with a permanent magnet 3 o 4 Solid, the obtained solid was washed three times with water by pouring method, then washed three times with ethanol, and dried in vacuum.

Embodiment 2

[0059] Fe 3 o 4 @SiO 2 Preparation of (FITC):

[0060] 15mg Fe at room temperature 3 o 4 Dissolve the powder in 231ml cyclohexane, add 60g Triton X-100, 48ml n-hexanol, 10.2ml H 2 O. 1.2ml tetraethyl orthosilicate (TEOS), stirred for 6 hours. Then add 3ml ammonia water, react for 24 hours, add 900 μl TEOS and 600 μl FITC-APS ethanol solution, and stir for 24 hours. Separation of red-brown Fe from reaction solution by permanent magnet 3 o 4 @SiO 2 (FITC) solid, the obtained solid was washed five times with absolute ethanol and once with absolute methanol by pouring method, and then dispersed in 40ml of absolute methanol.

Embodiment 3

[0062] Preparation of ICP-PAMAM:

[0063] Dissolve 91 mg of 2.0-generation polyamidoamine (PAMAM) dendrimers in 6 ml of anhydrous methanol at room temperature, add 7 μl (0.028 mmol) of isocyanatopropyltriethoxysilane (ICP) under magnetic stirring, and protect from light Stir for 24 hours.

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Abstract

The invention relates to a silica-coated fluorescent magnetic nanoparticle modified by amino or ester dendrimer. A superparamagetism Fe3O4 inner core is prepared by using a coprecipitation method; Fe3O4 encapsulated by silicon dioxide (Fe3O4 and SiO2) is formed by alkaline hydrolyzation of tetraethoxysilane (TEOS); and a fluorescent dye (fluorescein isothiocyanate FITC) is encapsulated in the silicon dioxide layer in a covalent way so as to facilitate tracing a carrier or to be taken as a model medicament. The carrier can be used for fluorescent tracing; and the external surface of the carrier is coated with polyamide-amine (PAMAM) dendrimer, so that the carrier has a great amount of functional groups used for modifying and used for connecting antibodies easier to target a blood brain barrier, and has the potential of becoming a targeted medicament carrier. Research results show that the two materials are injected through arteria carotis communis and can pass through the blood brain barrier. The ester silica-coated fluorescent magnetic nanoparticle is easier to pass through the blood brain barrier than the amino silica-coated fluorescent magnetic nanoparticle. In addition, the carrier can also be used for marking cells.

Description

technical field [0001] The invention belongs to the field of gene and drug transfer, in particular to a silicon-shell fluorescent magnetic nanoparticle. [0002] The present invention also relates to a preparation method of the above silicon-shell fluorescent magnetic nanoparticles. [0003] The present invention also relates to the application of the silicon-shell fluorescent magnetic nanoparticles. Background technique [0004] The existence of the blood-brain barrier has become a bottleneck in the treatment of central nervous system diseases. Neurosurgical methods such as intracerebral intubation and intraventricular injection, which are commonly used clinically, are likely to cause intracranial infection and are not conducive to long-term medication. Therefore, the study of drug transport across the blood-brain barrier using non-craniotomy has increasingly become a research hotspot in the treatment of brain diseases. Nanoparticles have a strong adsorption force to the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/02C09K11/06H01F1/10A61K47/48A61P25/00A61K47/59
Inventor 叶玲于景娴王楠杨慧赵焕英王迎库姝婷高琳
Owner CAPITAL UNIVERSITY OF MEDICAL SCIENCES
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