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Application of eupatorium adenophorum vinegar liquid as plant nematocide

A technology of Eupatorium adenophorum and vinegar liquid, which is applied in the direction of nematicides, biocides, plant growth regulators, etc., can solve the problems of serious pollution of human living environment and high cost of use, and achieve the effect of high-efficiency nematicide activity

Inactive Publication Date: 2012-11-07
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention overcomes the high cost of using chemical agents to prevent and control plant pathogenic nematodes in the prior art, and seriously pollutes the defects of the human living environment, reduces the harm of harmful plants to the ecological environment, and provides Eupatoria adenophorum vinegar liquid for killing New uses of plant pathogenic nematodes

Method used

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  • Application of eupatorium adenophorum vinegar liquid as plant nematocide
  • Application of eupatorium adenophorum vinegar liquid as plant nematocide
  • Application of eupatorium adenophorum vinegar liquid as plant nematocide

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0024] Example 1 Eupatorium adenophorum vinegar and its preparation

[0025] Eupatoria adenophorum vinegar via figure 1 made of equipment, figure 1 The equipment shown is a conventional simple preparation device for vinegar liquid designed by using the dry distillation and condensation principle of thermal decomposition. The components are connected and installed according to the conventional connection and installation methods of laboratory instruments. The round bottom flask 11 is filled with distilled water. The condensed exhaust gas is purified and filtered. The Latin literary name of the Eupatorium adenophorum plant used below is Eupatorium adenophorum Spreng.

[0026] 1, Eupatorium adenophorum vinegar liquid, described Eupatorium adenophorum vinegar liquid is prepared by the following method:

[0027] a. Cut Eupatorium adenophorum into small sections, and add it to retort 1;

[0028] b. Add the sealing ring on the retort 1, install the cover of the retort 1, and tigh...

example 2

[0046] Example 2: Nematicidal Activity Test of Eupatorium adenophorum Vinegar Liquid on Pine Xylophilus

[0047] Test reagent: 150ml of the Eupatorium adenophorum vinegar solution obtained by the preparation method of Example 1 was prepared into 25 times of liquid, 50 times of liquid, 75 times of liquid, 100 times of liquid, and 150 times of liquid according to the 6 dilution gradients set. Double liquid and 200 times liquid preparation solution.

[0048] Test method: Select a high-quality transparent watch glass (diameter 60mm), add 100 μl (300 pieces / ml) of pine wood nematode (Bursaphelenchus xylophilus) suspension into each watch glass with a pipette gun, and then add 2ml Adenophora adenophora to the watch glass For the preparation solution of blue vinegar liquid, use the original solution to correct the soaking solution in the watch glass, place the watch glass in a petri dish, and place it in a constant temperature incubator at 26°C for cultivation. At 12h, 24h, 48h, and...

example 3

[0055] Example 3: Nematicidal Activity Test of Eupatorium adenophorum Vinegar Liquid on Pine Xylophilus

[0056] Except adding 100 μ l (300 pieces / ml) of pine wood nematode (Bursaphelenchus mucrona tus) worm suspension with a pipette gun in each watch glass described in embodiment 2, all the other test methods and test reagents, reagent set 6 Each dilution gradient etc. are all the same as in Example 2, and will not be repeated.

[0057] Test results: Eupatorium adenophorum vinegar solution with different dilution concentrations had inhibitory effect on B. xylophilus, but the inhibitory effect decreased with the increase of dilution multiple. Under the same dilution factor, the inhibitory effect of Eupatorium adenophorum vinegar was gradually strengthened as time went on (Table 2).

[0058] Table 2 Nematicidal activity of Eupatorium adenophorum vinegar solution against pine wood nematode

[0059]

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Abstract

The invention discloses an application of eupatorium adenophorum vinegar liquid as plant nematocide, belonging to the technical field of botanical pesticide research and development. The eupatorium adenophorum vinegar liquid is prepared by the following method: cutting the eupatorium adenophorum into small sections to be added into a retort; heating the retort 1; condensing and collecting all fractions at the temperature of 90 DEG C-300 DEG C under normal pressure; and obtaining the collected liquid, namely the eupatorium adenophorum vinegar liquid. The application of the eupatorium adenophorum vinegar liquid as plant nematocide shows that: the eupatorium adenophorum vinegar liquid has highly effective nematicidal activity to plant nematodes, and the nematocide effect of 150-25 times of the eupatorium adenophorum vinegar liquid can achieve 70% to 100%.

Description

technical field [0001] The invention relates to the research on the activity of killing plant nematodes by using Eupatorium adenophorum vinegar liquid, and belongs to the technical field of development and research of botanical pesticides for preventing and controlling plant pathogenic nematodes. Background technique [0002] Plant pathogenic nematodes (plant nematodes for short) are important diseases with soil-borne characteristics, and it is difficult to control them. The application of chemical agents is still one of the main measures to prevent and control plant pathogenic nematodes, but there are still defects such as high cost of use and serious pollution of human living environment. [0003] Eupatorium adenophorum Spreng (Eupatorium adenophorum Spreng) inhibits the growth of other plants around it because it invades farmland and woodland, and soon forms a monodominant community, so it has become a harmful organism in my country and has caused huge economic losses. T...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N65/12A01P5/00
Inventor 胡先奇李维蛟李强杨艳丽王扬陈志星田野吕明涛裴卫华张晓云
Owner YUNNAN AGRICULTURAL UNIVERSITY
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