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Stewartia sinensis herba lycopi vinegar liquid and preparation method thereof

A technology of Eupatorium adenophorum and vinegar liquid, which is applied in the field of research and development of botanical pesticides, can solve the problems of cumbersome extraction of active substances of Eupatorium adenophorum, high investment costs, harm to human body and environment, etc., and achieve high nematicide activity, The effect of simple process and low cost

Inactive Publication Date: 2013-01-30
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to overcome the defects that there is no Eupatoria adenophorum vinegar liquid product in the prior art, the extraction process of the active substance of Eupatorium adenophorum is cumbersome, the investment cost is large, and the organic reagent used in the extraction process is harmful to the human body and the environment. , provide a kind of eupatorium adenophorum vinegar liquid and its simple preparation method, this eupatorium adenophorum vinegar liquid has the activity of killing plant nematodes efficiently

Method used

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  • Stewartia sinensis herba lycopi vinegar liquid and preparation method thereof
  • Stewartia sinensis herba lycopi vinegar liquid and preparation method thereof
  • Stewartia sinensis herba lycopi vinegar liquid and preparation method thereof

Examples

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Effect test

example 1

[0032] Example 1 Eupatorium adenophorum vinegar liquid of the present invention and preparation method thereof

[0033] Eupatorium adenophorum vinegar liquid of the present invention passes through figure 1 made of equipment, figure 1 The shown equipment is a conventional simple preparation device for vinegar liquid designed by utilizing the principle of dry distillation and condensation. All parts are connected and installed according to the conventional connection and installation methods of laboratory instruments. Among them, distilled water is filled in the round bottom flask 10, and the uncondensed tail gas Perform purification filtration.

[0034] The Latin literary name of the Eupatorium adenophorum plant used below is Eupatorium adenophorum Spreng.

[0035] (1) Eupatorium adenophorum vinegar liquid of the present invention, described Eupatorium adenophorum vinegar liquid is prepared by following method:

[0036] a. Cut Eupatorium adenophorum into small sections, and...

Embodiment 2

[0055] Example 2 Nematicidal activity test of Eupatorium adenophorum vinegar liquid of the present invention on pine wood nematode

[0056] Test reagent: use 150ml of the Eupatorium adenophorum vinegar liquid of the above-mentioned embodiment 1, and prepare 25 times of liquid, 50 times of liquid, 75 times of liquid, 100 times of liquid, 150 times of liquid, and 200 times of liquid according to the 6 dilution gradients set prepared solution.

[0057] Test method: Select a high-quality transparent watch glass (diameter 60mm), add 100 μl (300 pieces / ml) of pine wood nematode (Bursaphelenchus xylophilus) suspension into each watch glass with a pipette gun, and then add 2ml Adenophora adenophora to the watch glass Prepare the solution with blue vinegar solution, use the original solution to correct the soaking solution in the watch glass, put the watch glass in a petri dish, and put it in a constant temperature incubator at 26°C for cultivation. At 12h, 24h, 48h, and 72h respectiv...

Embodiment 3

[0064] Example 3 Nematicidal Activity Test of Eupatorium adenophorum Vinegar Liquid of the Present Invention to Meloidogyne incognita

[0065] Except adding 100 μ l (300 pieces / ml) of root-knot nematode (Meloidogyne incognita) 2nd instar larvae suspension with a pipette gun in each watch glass described in embodiment 2, all the other test methods and test reagents, reagent settings The 6 dilution gradients are the same as those in Example 2 and will not be repeated here.

[0066] Test results: Eupatorium adenophorum vinegar solution with different dilution concentrations had inhibitory effects on the 2nd instar larvae of Meloidogyne incognita, but the inhibitory effect decreased with the increase of dilution times. Under the same dilution factor, the inhibitory effect of Eupatorium adenophorum vinegar was gradually strengthened as time went on (Table 2).

[0067] Table 2 Nematicidal activity of Eupatorium adenophorum vinegar against root-knot nematode incognita

[0068]

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Abstract

The invention discloses a stewartia sinensis herba lycopi vinegar liquid and a preparation method thereof, belonging to the technical field of development and research of plant source pesticide. The preparation method of the stewartia sinensis herba lycopi vinegar liquid comprises the following steps: cutting stewartia sinensis herba lycopi into short sections, adding the sections into a dry distillation kettle, heating the dry distillation kettle, condensing, collecting all distillates at the temperature of 90-300 DEG C and collecting liquid of the stewartia sinensis herba lycopi vinegar liquid of the invention at 12h+ / -20min. The stewartia sinensis herba lycopi vinegar liquid has an effective nematode-killing activity against plant nematodes, and the plant nematode killing effect of liquid with 25-150 times of the vinegar liquid can reach 70-100%. The preparation method has simple technique; and compared with methods of extracting by organic solvents, the preparation method has the advantages of lower cost and environment protection.

Description

technical field [0001] The invention relates to Eupatoria adenophorum vinegar prepared by pyrolysis and a preparation method thereof, belonging to the technical field of development and research of botanical pesticides. Background technique [0002] Eupatorium adenophorum Spreng (Eupatorium adenophorum Spreng) inhibits the growth of other plants around it because it invades farmland and woodland, and soon forms a monodominant community, so it has become a harmful organism in my country and has caused huge economic losses. Due to the destructiveness of Eupatorium adenophorum, complete eradication is the best preventive measure, but in areas where serious damage has occurred, research and development of its potential utilization value is also an important measure to reduce its negative impact. [0003] With the continuous research on Eupatorium adenophorum, the utilization of Eupatorium adenophorum has gradually increased, and has brought certain economic benefits and utilizat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N65/12A01P5/00
Inventor 李维蛟胡先奇杨艳丽李强王扬陈志星田野吕明涛裴卫华张晓云
Owner YUNNAN AGRICULTURAL UNIVERSITY
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