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Carcinoembryonic antigen positive cell targeted gene expression element CPE and application thereof

A gene expression, positive cell technology, applied in gene therapy, antineoplastic drugs, DNA/RNA fragments, etc., can solve the problem of unsatisfactory effect of blocking gene expression, and achieve the effect of inhibiting proliferation and causing cell cycle block.

Inactive Publication Date: 2010-02-03
杭州安倍生物科技有限公司
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  • Abstract
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AI Technical Summary

Problems solved by technology

In a large number of previous studies, people used antisense RNA (antisense RNA) to block highly expressed genes in tumor cells. Although great achievements have been made, the effect of closing gene expression is still unsatisfactory

Method used

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  • Carcinoembryonic antigen positive cell targeted gene expression element CPE and application thereof
  • Carcinoembryonic antigen positive cell targeted gene expression element CPE and application thereof
  • Carcinoembryonic antigen positive cell targeted gene expression element CPE and application thereof

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Embodiment 1

[0029] Cloning of regulatory sequences expressed by human CEA positive cells:

[0030] Firstly, the genomic DNA sequence including the 5' flank and the first exon of the human CEA gene was retrieved from GenBank (GenBank accession number: Z21818), and then referred to the literature of Nyati MK et al. (Cancer Research 62, 2337-2342, 2002) , design and synthesize primer 1 and primer 2, and use polymerase chain reaction (Polymerase Chain Reaction, PCR) to amplify the 5' upstream of the CEA translation initiation point (designated as +1) containing the basic promoter and part of the first exon Sequence at positions -407 to -39 including the untranslated region. The sequence of primer 1 is: 5'-AGA TCT CCC GGG ACC CTG CTG GGT TTC-3', the sequence of primer 2 is: 5'-GGA TCC AGC TTG AGT TCC AGG AAC G-3', human intestinal cancer cell SW620 genomic DNA As a template, the above primers were used for nucleic acid amplification to obtain a 381bp amplification product.

[0031] The above...

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Abstract

The invention relates to a novel tumor targeted gene expression element, named CPE. The element as a DNA molecule can be specifically expressed in a carcinoembryonic antigen positive tumor cell and can effectively block DNA replication in the tumor cell so that a cell cycle can not be carried out due to DNA replication blockage, and the effect of specifically inhibiting tumor cell proliferation can be achieved. The invention can be used for preparing a tumor gene treating medicine.

Description

technical field [0001] The present invention belongs to biological technology, and relates to regulation of gene expression, in particular, relates to the design and preparation of a targeted gene expression element CPE specific for carcinoembryonic antigen-positive tumor cells and its incorporation into carcinoembryonic antigen-positive tumor cells. Specifically expressed, its product can effectively block the DNA replication in tumor cells, so that the cell cycle cannot continue due to the blockage of DNA replication, thereby achieving the effect of inhibiting cell proliferation. The invention can be used to prepare targeted tumor gene therapy drugs. Background technique [0002] Malignant tumor is currently one of the main "killers" of human beings. With the change of people's lifestyle and environment in recent years, its incidence is on the rise. It has become the first cause of death in many cities and the second in rural areas. Lung cancer, gastrointestinal tumors, a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11A61K48/00A61P35/00C12N15/113
Inventor 曹江贾振宇
Owner 杭州安倍生物科技有限公司
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