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Carcinoembryonic antigen positive cell targeted gene expression element CPD and application thereof

A gene expression and positive cell technology, applied in gene therapy, anti-tumor drugs, recombinant DNA technology, etc., can solve the problem of unsatisfactory closed gene expression

Inactive Publication Date: 2010-02-03
杭州安倍生物科技有限公司
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

In a large number of previous studies, people used antisense RNA (antisense RNA) to block highly expressed genes in tumor cells. Although great achievements have been made, the effect of closing gene expression is still unsatisfactory

Method used

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  • Carcinoembryonic antigen positive cell targeted gene expression element CPD and application thereof
  • Carcinoembryonic antigen positive cell targeted gene expression element CPD and application thereof
  • Carcinoembryonic antigen positive cell targeted gene expression element CPD and application thereof

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Embodiment 1

[0029] Cloning of regulatory sequences expressed by human CEA positive cells:

[0030] Firstly, the genomic DNA sequence including the 5' flank and the first exon of the human CEA gene was retrieved from GenBank (GenBank accession number: Z21818), and then referred to the literature of Nyati MK et al. (Cancer Research 62, 2337-2342, 2002) , design and synthesize primer 1 and primer 2, and use polymerase chain reaction (Polymerase Chain Reaction, PCR) to amplify the 5' upstream of the CEA translation initiation point (designated as +1) containing the basic promoter and part of the first exon Sequence at positions -407 to -39 including the untranslated region. The sequence of primer 1 is: 5'-AGA TCT CCC GGG ACC CTG CTG GGT TTC-3', the sequence of primer 2 is: 5'-GGA TCC AGC TTG AGT TCC AGG AAC G-3', human intestinal cancer cell SW620 genomic DNA As a template, the above primers were used for nucleic acid amplification to obtain a 381bp amplification product.

[0031] The above...

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Abstract

The invention belongs to biotechnologies and relates to regulation and control of gene expression, in particular to design and preparation of a targeted gene expression element CPD specifically aimingat a carcinoembryonic antigen positive tumor cell and specific expression in the carcinoembryonic antigen positive tumor cell. A product can effectively block DNA replication in the tumor cell so that a cell cycle can not be carried out due to the blockage of the DNA replication, and the effect of inhibiting cell proliferation can be achieved. The invention can be used for preparing a targeted tumor gene treating medicine. The invention has the advantages of favorable tumor inhibiting effect, wide adaptability and high safety.

Description

technical field [0001] The present invention belongs to biological technology, and relates to regulation of gene expression, in particular, relates to the design and preparation of a targeted gene expression element CPD specific for carcinoembryonic antigen-positive tumor cells and its incorporation into carcinoembryonic antigen-positive tumor cells. Specifically expressed, its product can effectively block the DNA replication in tumor cells, so that the cell cycle cannot continue due to the blockage of DNA replication, thereby achieving the effect of inhibiting cell proliferation. The invention can be used to prepare targeted tumor gene therapy drugs. Background technique [0002] Malignant tumor is currently one of the main "killers" of human beings. With the change of people's lifestyle and environment in recent years, its incidence is on the rise. It has become the first cause of death in many cities and the second in rural areas. Lung cancer, gastrointestinal tumors, a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11A61K48/00A61P35/00C12N15/113
Inventor 曹江贾振宇
Owner 杭州安倍生物科技有限公司
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