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Rana pleuraden antioxidant peptide and genes and application thereof

An anti-oxidative peptide, Yunnan frog technology, applied in the field of biomedicine, to achieve the effects of convenient artificial synthesis, simple structure and strong anti-inflammatory activity

Active Publication Date: 2010-02-03
KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

At present, there are few reports on the research on active substances in the skin of Dianna frog

Method used

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Embodiment Construction

[0026] The substantive content of the present invention is further described below with embodiment, but content of the present invention is not limited thereto.

[0027] Cloning of Antioxidant Peptide Gene of Dianna Frog:

[0028] I. Extraction of total RNA from Dianna frog skin:

[0029] A. The live Dian frog was cleaned with water, put into liquid nitrogen and quick-frozen for 4 hours, took the skin tissue, weighed, got 300mg skin tissue, added 10ml total RNA extraction buffer (Trizol solution, the product of GIBCOBRL company in the United States), and put it in 20ml glass Homogenize in a homogenizer for 30 minutes.

[0030] B. Add an equal volume of phenol / chloroform solution, shake and mix well, let stand at room temperature for 10 minutes, centrifuge at 12,000 rpm for 10 minutes at 4°C, and discard the precipitate.

[0031] C. Add an equal volume of isopropanol to the supernatant, leave it at room temperature for 10 minutes, centrifuge at 12,000 rpm at 4°C for 10 minute...

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Abstract

The invention relates to rana pleuraden antioxidant peptide and genes and an application thereof, belonging to the technical field of biomedical science. The rana pleuraden antioxidant peptide is thesingle chain polypeptide through genetic encoding of Chinese amphibians rana pleuraden, wherein, the molecular weight is 1653.88 daltons, the isoelectric point is 8.22, and the complete sequence of the rana pleuraden antioxidant peptide is NH2-GIRPTYNRQCEIGF-COOH; the gene for encoding is composed of 316 nucleotides, wherein, the encoding mature part is the 130-171th nucleotide. The synthetic ranapleuraden antioxidant peptide features strong antioxidant activity, can be applicable to the preparation of medicines of skin antioxidant protection and free radicals in vivo elimination, and has theadvantages of simple sequence and convenient synthesis.

Description

Technical field: [0001] The invention provides a Rana pleuraden antioxidant peptide and its gene and application, belonging to the technical field of biomedicine. Background technique: [0002] In traditional Chinese medicine and ethnic medicine in China, many amphibians are widely used as medicinal materials, such as Chinese toad (Bufo gargarizans), large webbed bell toad (Bombina maxima), black-spotted frog (pelophylax nigromaculata), marsh frog (Hylaranaguentheri) and the frog (Euphlyctislimnocharis) and so on. The skin and internal organs of these amphibians have a wide range of pharmacological activities and clinical curative effects. The reported pharmacological activities include: broad-spectrum antibacterial effect, anti-tumor, local anesthesia, analgesia, immune regulation, and effects on the cardiovascular system. On the one hand, the complexity of traditional Chinese medicine ingredients and the limitations of their processing methods are also important reasons f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/08C12N15/12A61K38/10A61P39/06A61P17/18
Inventor 赖仞杨海龙王旭刘秀红武静刘存宝
Owner KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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