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High-purity salvianolic acid B and preparation method and application thereof

A salvianolic acid, high-purity technology, applied in the field of high-purity salvianolic acid B, preparation and application, can solve the problems of unsuitable industrial production, low purity of salvianolic acid B, and many steps, and achieve low cost and low price , The effect of simple process

Inactive Publication Date: 2010-02-03
王宇
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of the prior art is that the purity of salvianolic acid B obtained is lower than 92%, and there are many steps to obtain more than 92% salvianolic acid B, the cost is very high, and it cannot be used for industrial production

Method used

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  • High-purity salvianolic acid B and preparation method and application thereof

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Embodiment 1

[0031] Take 1000g of Salvia miltiorrhiza, grind it, add deionized water, heat and extract at 100°C for 1 hour, use water 5 times the weight of Salvia miltiorrhiza, and decoct and extract twice under the condition of pH 4.8, 40 minutes each time, use the weight of Danshen 3 times water. The extract was filtered, the pH was adjusted to 3, and the DA-201 polar resin was used for chromatography, and the impurities were eluted with water, and then 10% ethanol was used for removal of impurities, and 80% ethanol was used for desorption. The components containing salvianolic acid B were collected and recovered under reduced pressure. The resulting solution was then chromatographed on a polyamide column and desorbed with 95% ethanol to obtain components containing salvianolic acid B. The fractions with a purity greater than 98.5% were collected in sections, concentrated under reduced pressure, and adjusted to pH 4.8. The concentrated solution is passed through a Sephadex column, and t...

Embodiment 2

[0034] Take 1500g of Salvia miltiorrhiza, grind it, add deionized water, heat and extract at 100°C for 1 hour, use 5 times of water, then decoct and extract 3 times under the condition of pH4.8, each time for 40 minutes, use 3 times of water . The extract was filtered, the pH was adjusted to 2, and the DA-201 polar resin was used for chromatography, and the impurities were eluted with water, and then 20% ethanol was used for removal of impurities, and 90% ethanol was used for desorption. The components containing salvianolic acid B were collected and recovered under reduced pressure. The resulting solution was then chromatographed on a polyamide column and desorbed with 90% ethanol to obtain components containing salvianolic acid B. The fractions with a purity greater than 98% were collected in sections, concentrated under reduced pressure, and adjusted to pH 5. The concentrated solution is passed through a Sephadex column, and the parts with a purity greater than 98.5% are c...

Embodiment 3

[0036] Take 2000g of Salvia miltiorrhiza, grind it, add deionized water, heat and extract at 100°C for 1 hour, use 5 times the water, and then decoct and extract twice under the condition of pH4.8, each time for 40 minutes, use 3 times of water. Filter the extract, adjust the pH to 4, use DA-201 polar resin chromatography, elute with water to remove impurities, then remove impurities with 10% ethanol, desorb with 95% ethanol, and collect the group containing salvianolic acid B Separate, and recover ethanol under reduced pressure. The obtained product is then chromatographed on a polyamide column, desorbed with 95% ethanol, and the parts with a purity greater than 98% are collected in sections, concentrated under reduced pressure, and adjusted to pH 4.8. The concentrated solution is passed through a Sephadex column, and the parts with a purity greater than 98.5% are collected in sections, concentrated under reduced pressure, and freeze-dried to obtain high-purity salvianolic ac...

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Abstract

The invention relates to a high-purity salvianolic acid B and a preparation method and application thereof. The defects of the prior art are that the purity of the obtained salvianolic acid B is lowerthan 92 percent, the steps for obtaining the salvianolic acid B of which the purity is above 92 percent are quite a lot, the cost is very high, and the prior art cannot be applied to industrial production. The preparation method consists of the steps of extraction, pre-column, polyamide chromatography and collection, wherein the pre-column comprises the following steps: filtering an extracting solution and then putting the same on a DA-201 type polar resin column, and using water to remove impurities firstly; then using low-concentration lower alcohol with 1 to 5 carbon atoms to remove the impurities; using the lower alcohol with 1 to 5 carbon atoms to perform desorption; collecting the components containing the salvianolic acid B section by section; and performing decompression and concentration. The salvianolic acid B is applied to the medicaments for treating cardiovascular and cerebrovascular diseases, kidney diseases, pneumonia, pulmonary heart diseases, pancreatitis, diabetes, cervical spondylosis, retinal vascular diseases, retinal nerve diseases, migraine headaches, chronic gastritis, dizziness, bone diseases, and Alzheimer's diseases.

Description

Technical field: [0001] The invention relates to a method for extracting and isolating salvianolic acid B from Chinese medicinal material Salvia miltiorrhiza and the application of salvianolic acid B. Background technique: [0002] Salvia miltiorrhiza Bge, a plant of the Labiatae family, is a perennial herb that has the effects of dispelling blood stasis and relieving pain, promoting blood circulation and stimulating menstruation, and clearing heart and annoyance. In modern clinical practice, it is widely used to prevent and treat cardiovascular and cerebrovascular diseases, kidney diseases, liver diseases, etc. Due to the complex ingredients in the extract, it is difficult to promote it to the world. [0003] Salvianolic acid B is a water-soluble phenolic acid isolated from Salvia miltiorrhiza, and its molecular formula is C 36 h 30 o 16 , the molecular weight is 718.614, and the structural formula is: [0004] [0005] Clinical medicine has proved that salvianolic ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D307/84A61K31/343A61P9/00A61P11/00A61P13/12A61P27/02A61P25/28A61K36/53
Inventor 王宇
Owner 王宇
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