Method for preparing biopesticide tetramycin and application
A technology of Wuningmycin and bio-pesticides, applied in the field of application in the prevention and treatment of apple spot leaf defoliation, can solve the problems of limited use and efficacy, unsatisfactory control effect of apple rot disease, and high cost
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Embodiment 1
[0102] Embodiment 1: the preparation of Wuningmycin fermented liquid
[0103] The present invention passes through the preferred culture medium formula of orthogonal test:
[0104] (1) Seed fermentation medium: every 100 ml contains: soybean cake powder 1.0g, starch 1.0g, glucose 0.7g, peptone 0.5g, magnesium sulfate 0.03g, ammonium sulfate 1.0g, dipotassium hydrogen phosphate 0.02g, Calcium carbonate 0.7g, sodium chloride 0.1g, vegetable oil 0.2g, pH natural;
[0105](2) Submerged fermentation medium: every 100 ml contains: glucose 2.5g, starch 1.5g, cornmeal 1.0g, soybean cake powder 2.0g, peptone (or corn steep liquor) 0.5g, ammonium sulfate 0.2g, magnesium sulfate 0.03g, 0.02g of dipotassium hydrogen phosphate, 0.7g of calcium carbonate, 0.1g of sodium chloride, 0.2g of vegetable oil, after sterilization, add 0.4% of the medium volume of n-propanol, and the pH is natural.
[0106] The present invention has optimized fermentation condition:
[0107] (1) Preparation of se...
Embodiment 2-5
[0109] Example 2-5: Application examples of Wuningmycin in the prevention and treatment of apple spot defoliation disease
[0110]
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