Nanometer molecular biosensor, preparation method and application thereof

Abstract

The invention discloses a nanometer molecular biosensor and a preparation method thereof. The biosensor is mainly characterized in that a molecular biosensor is self-assembled into a nanometer molecular biosensor by adopting nanowire protein, and compared with an unassembled molecular biosensor, the detection sensitivity is greatly improved. The preparation method adopts the protein fusion technology, and fuses nanowire protein with self-assembled function, special enzyme molecule used as a biology identification element and fluorescence protein used as a transducer element, therefore, functional protein with self-assembled nanowire, identification and transduction output signal functions simultaneously, and after the functional protein is expressed and purified, the functional protein is self-assembled into a nanometer molecular biosensor which can be used for inspecting object molecules in various clinical samples and environment samples. The invention simultaneously discloses an application of the nanometer molecular biosensor prepared by the preparation method in pesticide methyl parathion detection.

Description

technical field [0001] The invention belongs to the technical fields of molecular biology and analytical biology, and in particular relates to a nano-molecular biosensor, a preparation method of the nano-molecular biosensor, and an application of the nano-molecular biosensor. Used to detect target molecules in various clinical and environmental samples. Background technique [0002] Biosensors use certain biological or chemical immobilization techniques to immobilize biological recognition elements (enzymes, antibodies, antigens, etc.) Transform the generated reaction results into electrical and optical signals that can be output and detected, so as to conduct qualitative and quantitative analysis of the substance to be tested, so as to achieve the purpose of detection and analysis. As far as the current development of biosensors is concerned, the mainstream is still electrochemical biosensors, which use some substances with biological characteristics, such as enzymes, anti...

AI Technical Summary

Problems solved by technology

Although the toxicity of methyl parathion is only equivalent to 1 / 3 of that of parathion, it is still highly toxic and has systemic properties. Therefore, it is necessary to apply pesticides strictly according to regulations and strengthen safety protection measures

However, incidents of methyl parathion poisoning still occur from time to time

Especially its extensive use on crops will cause great harm to human health

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