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Inorganic phosphorus (phosphate radical) diagnosis/determination kit and method for determining inorganic phosphorus (phosphate radical) concentration

A technology of phosphate and inorganic phosphorus, applied in the field of determination of inorganic phosphorus concentration, inorganic phosphorus diagnosis/measurement kit, can solve the problem of high results

Inactive Publication Date: 2010-01-06
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, jaundice, hemolysis, and fat-turbid serum have light absorption at 340nm, and a blank specimen must be prepared, otherwise the result will be high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] The inorganic phosphorus (phosphate) diagnostic / measurement reagent in this embodiment is a single reagent, including:

[0046] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0047] Stabilizer 500mmol / L

[0048] Coenzyme 3mmol / L

[0049] Phosphoenolpyruvate carboxylase 10000U / L

[0050] Glutamate decarboxylase 12000U / L

[0051] Glutamate synthase 12000U / L

[0052] Oxaloacetic acid 12mmol / L

[0053] 4-aminobutyric acid 9mmol / L

[0054] After all the reagents are dissolved and prepared, they are divided into bottles and freeze-dried to prepare dry powder reagents; before use, add pure water and use after reconstitution.

[0055] Set on the automatic biochemical analyzer: temperature 37℃, reaction time 10 minutes, initial absorbance ≤0.1, test main wavelength 340nm, test sub-wavelength 405nm, the volume ratio of the tested inorganic phosphorus (phosphate) sample to the reagent is 1 / 25, the reaction direction is positive reaction (rising reaction), the delay time is...

Embodiment 2

[0058] The inorganic phosphorus (phosphate) diagnostic / measurement reagent in this embodiment is a dual reagent, including:

[0059] Reagent 1

[0060] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0061] Stabilizer 50mmol / L

[0062] Coenzyme 3mmol / L

[0063] Oxaloacetic acid 12mmol / L

[0064] 4-aminobutyric acid 9mmol / L

[0065] Reagent 2

[0066] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0067] Stabilizer 50mmol / L

[0068] Phosphoenolpyruvate carboxylase 10000U / L

[0069] Glutamate decarboxylase 12000U / L

[0070] Glutamate synthase 12000U / L

[0071] After all the reagents are dissolved and prepared, they are divided into bottles to make a liquid double reagent, which can be used directly.

[0072] Set on the automatic biochemical analyzer: temperature 37℃, reaction time 10 minutes, initial absorbance ≤0.1, test main wavelength 340nm, test sub-wavelength 405nm, tested inorganic phosphorus (phosphate) sample and reagent 1, reagent 2 The volume r...

Embodiment 3

[0075] The inorganic phosphorus (phosphate) diagnostic / measurement reagents in this embodiment are three reagents, including:

[0076] Reagent 1

[0077] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0078] Stabilizer 50mmol / L

[0079] Coenzyme 3mmol / L

[0080] Oxaloacetic acid 12mmol / L

[0081] 4-aminobutyric acid 9mmol / L

[0082] Reagent 2

[0083] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0084] Stabilizer 500mmol / L

[0085] Glutamate decarboxylase 12000U / L

[0086] Glutamate synthase 12000U / L

[0087] Reagent 3

[0088] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0089] Stabilizer 500mmol / L

[0090] Phosphoenolpyruvate carboxylase 10000U / L

[0091] After all the reagents are dissolved and prepared, they are divided into bottles to form three liquid reagents, which can be used directly.

[0092] When determining the concentration of inorganic phosphorus (phosphate), set on the automatic biochemical analyzer: temperature 3...

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Abstract

The invention relates to an inorganic phosphorus (phosphate radical) diagnosis / determination kit using the technology of an enzyme colorimetric method and an ELISA method, a method for determining inorganic phosphorus (phosphate radical) concentration and composition and components of a reagent, and belongs to the technical field of test and determination of medical science, food, and environment. The kit comprises the following main components: buffer solution, coenzyme, oxaloacetic acid, 4-aminobutyric acid, phosphoenolpyruvate carboxykinase, glutamate dehydrogenase, glutamate sythetase and a stabilizer. The method comprises the following steps: mixing a sample and the reagent according to a certain volume ratio to perform a series of enzymic reaction, and then placing reactants under a UV / visible light analyzer to test the descending speed of absorbance at the position where the dominant wave length is 340nm to determine the inorganic phosphorus (phosphate radical) concentration.

Description

Technical field [0001] The present invention relates to an inorganic phosphorus (phosphate) diagnostic / measurement kit. At the same time, the present invention also relates to a method for determining the concentration of inorganic phosphorus (phosphate), belonging to the technical field of medical / food / environmental inspection and measurement. Background technique [0002] 87% of the phosphorus in the human body is present in the bones. The phosphorus in the blood and the phosphorus in the bones maintain a dynamic balance. There is a certain relationship between the concentration of calcium and phosphorus in the blood. The increase in blood calcium in normal people will reduce the blood phosphorus, and vice versa. Of course. The product of [calcium] and [phosphorus] concentrations in plasma is kept within a certain range; approximately the product of calcium and phosphorus concentrations per 100 ml of plasma is 3540. When the product of the two is greater than 40, calcium and p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N21/25G01N33/84C12Q1/00
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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