Production method of bacterial exotoxin

Abstract

The invention discloses a production method of bacterial exotoxin, comprising the procedures of bacterial toxin production culture, bacterial exotoxin extraction, bacterial exotoxin purification and bacterial exotoxin preservation, wherein the bacterial toxin production culture is carried out in a microorganism dialysis incubator which comprises a culture medium chamber and a culture chamber, a dialysis membrane and a dialysis membrane supporting and protecting device are arranged between the culture medium chamber and the culture chamber, the ratio of the volume of the culture medium chamber to the total volume of the culture chamber is selected between 3:1 and 1:6 according to a microorganism culture time, and facilities needed by microorganism growth are arranged in the culture medium chamber. The production method comprises the procedures of toxin production culture, sterilization, filtration, concentration, precipitation, percrystallization and preservation. The production method of bacterial exotoxin is adopted to produce toxin, optimizes bacterial growth environment, simplifies the technologies of concentration and purification of bacterial exotoxin, increases the yield to 90 percent from 17-33 percent, and shortens the purification time to about 12-24 hours from the original natural dialysis of 12-15 days.

Description

technical field [0001] The invention relates to a method for preparing microorganisms, in particular to a process for cultivating, extracting, concentrating and purifying bacterial exotoxins. Background technique [0002] Bacterial exotoxin is a macromolecular protein metabolized from the bacteria during the growth of bacteria. These macromolecular proteins have various virulence. At present, bacterial exotoxins have been applied in many fields such as medicine and pest control. For example, Clostridium botulinum type C vaccine and toxoid have been applied to the field of preventive medicine. In 1989, the US FDA officially approved type A botulinum toxin as a new drug and put it on the market. . At present, botulinum toxin type A has been applied in the fields of clinical medicine and cosmetic wrinkle removal, for example, it can be used in the treatment of dystonia diseases in neurology, ophthalmology and other fields - blepharospasm, hemifacial spasm, strabismus and spas...

AI Technical Summary

Problems solved by technology

However, the volume of the dialysis bag cannot be made too large, otherwise it is more likely to be damaged

Therefore, although dialysis can obtain highly active and high-quality bacteria and their derivatives, it is rarely used due to high cost and low success rate.

Although the dialysis culture method has been partially improved, the dialysis culture ratio of 10:1 is still used.

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