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Xylitol as well as preparation method and application thereof

A technology of xylitol and arabitol, which is applied in the fields of application, food science, tobacco processing, etc., and achieves the effects of simple operation, low equipment investment and operation cost, and strong continuous operability

Inactive Publication Date: 2010-01-06
HUABAO FLAVOURS & FRAGRANCES CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Enzymatic synthesis is likely to achieve continuous and efficient production. However, there are relatively few studies on enzymatic production of xylitol, and the technology needs to be further improved.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] The preparation method of xylitol of the present invention is as follows:

[0049] A. Dissolve d-arabitol in distilled water to prepare 1000g of a 40% by weight aqueous solution, adjust the pH of the aqueous solution to 6.5 with acetic acid, then add 10g of calcium carbonate and 30g of glucose to it, stir and mix evenly Sterilize for 10 minutes at a temperature of 120°C and a pressure of 0.1 MPa, and then cool down to room temperature;

[0050] B. Transfer the sterilized reaction liquid obtained in step A) to a bioreactor, and control the temperature of the bioreactor at 40°C under stirring at a stirring speed of 300rpm / min, and add arabinitol oxidative dehydrogenase 4.0g, at an air flow rate of 50mL / min, pass through the air purified by filtration with a micro-membrane filter, and carry out the enzymatic hydrolysis reaction for 12h;

[0051] C. Add 3.0 g of coenzyme NADH, 3.0 g of xylitol reductase, and 2.0 g of inducer xylitol to the enzymolysis reaction liquid obtai...

Embodiment 2

[0053] The preparation method of xylitol of the present invention is as follows:

[0054] A, d-arabitol is dissolved in distilled water and is mixed with concentration 35% by weight aqueous solution 1000g, adjusts the pH value of this aqueous solution to 7.0 with acetic acid, then adds 15g calcium carbonate and 30g glucose and fructose 1: 1 (weight ) mixture, stir and mix evenly, then sterilize at a temperature of 120°C and a pressure of 0.1MPa for 10 minutes, and then cool down to room temperature;

[0055] B. Transfer the sterilized reaction solution obtained in step A) to a bioreactor, and control the temperature of the bioreactor at 35°C under stirring at a stirring speed of 450rpm / min, and add arabinitol oxidative dehydrogenase 4.0g, at an air flow rate of 50mL / min, pass through the air purified by filtration with a micro-membrane filter, and carry out the enzymatic hydrolysis reaction for 12h;

[0056] C. Add 3.5 g of coenzyme NADH, 3.5 g of xylitol reductase, and 3.0 g...

Embodiment 3

[0058] The preparation method of xylitol of the present invention is as follows:

[0059] Carry out in the same manner as in Example 1, except that sucrose is used and 4.5 g of arabinitol oxidative dehydrogenase is added to obtain the xylitol product of the present invention. The product is determined by high-performance liquid chromatography, and its content reaches 99.6% by weight; its xylitol yield is 96% based on the weight of the substrate.

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PUM

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Abstract

The invention relates to a method for preparing xylitol by a complex enzyme method, the obtained xylitol and the application of the xylitol. The method is as follows: arabitol oxidizing dehydrogenase, xylitol reductase and inductive agent xylitol are added into d-arabitol, calcium carbonate and sugar so as to prepare the xylitol. The method has strong continuous operability, low equipment investment and operation cost, easy operation and industrialization realization and very high conversion rate and production rate of the xylitol. The xylitol prepared by the method can be used for tobacco products.

Description

【Technical field】 [0001] The present invention relates to the field of biotechnology. More specifically, the present invention relates to a method for preparing xylitol by using a compound enzyme method, also relates to the xylitol obtained by the method, and also relates to the use of the xylitol. 【Background technique】 [0002] Xylitol is a sugar that occurs naturally in nature. In recent years, due to its special functionality, the demand has been increasing. Xylitol has lower calories than sucrose, and its sweetness is comparable to sucrose, and xylitol has the effect of imitating dental caries. Recent studies have shown that xylitol can be used as a good humectant in cigarettes, which is more effective than traditional glycerin, sorbitol and other humectants. It also increases the sweetness and flavor of cigarettes and the softness of cigarettes. , reduces throat irritation, and improves the effect of cigarettes producing body fluids and returning to sweetness. [0...

Claims

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Application Information

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IPC IPC(8): C12P7/18C07C31/18A24B15/32
Inventor 谷向春张福军周小文
Owner HUABAO FLAVOURS & FRAGRANCES CO LTD
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