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A kind of canine distemper live vaccine and preparation method thereof

A canine distemper and live vaccine technology, which can be used in pharmaceutical formulations, medical preparations containing active ingredients, and antibody medical ingredients, etc., and can solve the problems of uneven immunogenicity of virus strains, immune failure, and low antigen titers.

Active Publication Date: 2011-12-07
QILU ANIMAL HEALTH PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Because canine distemper has the characteristics of sudden onset and difficulty in drug treatment, at present, it mainly relies on canine distemper vaccine for prevention. There are many vaccine products for the prevention of canine distemper at home and abroad, but in the actual application of disease prevention, due to There are problems such as the immunogenicity of the virus strains are uneven, and the antigen titer is not high. The vaccine used may have the problem of not being able to produce effective immune protection, resulting in immune failure.
At the same time, most of the canine distemper vaccine products at home and abroad are polyvalent live vaccines. If the farm has not had any diseases other than canine distemper, it may cause canine distemper. Risk of diseases other than heat, which is especially serious when using some products that are not prepared properly

Method used

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  • A kind of canine distemper live vaccine and preparation method thereof
  • A kind of canine distemper live vaccine and preparation method thereof
  • A kind of canine distemper live vaccine and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Purity Test of Virus Seeds

[0052] 1 Sterility test Inoculate the virus culture into two tubes of T.G, G.P small tubes and G.A slant medium respectively, each tube is 0.2ml, one is cultured at 37°C; one is cultured at 25°C, and observed for 3 days, the virus after transplantation The T.G, G.P small tubes and G.A slant medium inoculated with the cultures were observed for 5 days without bacteria and mold growth. Whether there is bacteria and mold growth.

[0053] 2. Mycoplasma test Take 5ml of virus culture, inoculate it into a vial of liquid mycoplasma culture medium, then get 0.8ml from the suspension in the vial and transplant it into 2 small test tubes of liquid mycoplasma culture medium and 2 pairs of agar solid plates. Liquid cultures were cultured at 37°C, solid cultures were placed at 37°C with 5% CO 2 Environmental cultivation. After culturing for 5, 10, and 15 days, take 0.6-0.8ml of the culture from the vial and transplant it into 2 small test tubes of liq...

Embodiment 2

[0059] manufacture of vaccines

[0060] 1 Preparation of cell nutrient solution

[0061] Conventional cell growth solution (containing 10% primary newborn bovine serum) and maintenance solution (containing 2% primary newborn bovine serum) were used for cell culture.

[0062] 2 Preparation of seed poison for production

[0063] Use the isolated natural attenuated strain of canine distemper to inoculate the Vero cell monolayer on the spinner bottle, the amount of inoculation is 2% of the maintenance solution, and it can be harvested when the cell lesion reaches 80%, and then passed on for one generation to prepare the production Use poison.

[0064] 3 Propagation of virus fluid

[0065] The seed virus for production was used to inoculate the Vero cell monolayer on the spinner bottle, and the inoculation amount was 2% of the cell maintenance solution. Then the spinner bottle cells were cultured at 37°C with a rotation speed of 8-10r / h.

[0066] After inoculation, observe the...

Embodiment 3

[0071] Finished product testing of vaccines.

[0072] (1) Sterility test should be sterile growth. According to the "Chinese Veterinary Pharmacopoeia" in the inspection.

[0073] (2) Mycoplasma inspection shall be carried out according to the regulations in "Chinese Veterinary Pharmacopoeia", and there should be no mycoplasma growth.

[0074] (3) Identification test The vaccine is 10 -2 diluted, with an equivalent of 10 -1 Diluted canine distemper positive serum was mixed, neutralized at 37°C for 30 minutes, inoculated into 4 bottles of Vero cells, and set up 2 bottles of virus control group at the same time, cultured at 37°C, and observed CPE continuously for 96-120 hours.

[0075] (4) Exogenous virus inspection shall be carried out according to the regulations in "Chinese Veterinary Pharmacopoeia", and there should be no exogenous virus.

[0076] (5) Safety inspection After dissolving each dose of vaccine with 1ml of normal saline, intramuscularly inoculate 5 susceptible...

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Abstract

The reagent of the invention is a canine distemper live vaccine and a preparation method thereof. The present invention uses a natural attenuated strain of canine distemper virus CGMCC No.3201 with excellent immunogenicity isolated from the field by the inventor as a production strain to prepare a safe, effective and single-component canine distemper virus live vaccines. The invention effectively reduces the risk of introducing other live viruses when immunizing the canine distemper vaccine, and provides conditions for controlling the spread of fur animal diseases.

Description

technical field [0001] The invention relates to a live canine distemper vaccine and a preparation method thereof, belonging to the technical field of veterinary biological products. Background technique [0002] Canine distemper (Canine Distemper, CD), commonly known as canine distemper, is a kind of fur animal such as mink, raccoon dog, fox and rare animals such as dogs and pandas and tigers caused by Canine Distemper virus (CDV) infection. Acute and severe infectious diseases are currently one of the most harmful diseases to my country's dog industry, fur animal breeding industry and wildlife protection industry. It is mainly popular in the cold seasons of winter and spring, and it is sporadic, endemic or outbreaks. Due to the ever-changing clinical manifestations of canine distemper, it is easy to be confused with canine distemper in clinical diagnosis, which brings certain difficulties to clinical diagnosis and experimental research. [0003] Canine distemper virus, bel...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61P31/14A61K39/175A61K39/295
Inventor 魏联果李秀娟徐龙涛王蕾禚宝山
Owner QILU ANIMAL HEALTH PROD
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