Axenic germination method of roegneria kamoji plants

Abstract

The invention belongs to the field of biotechnologies, in particular to an axenic germination and culture method of roegneria kamoji plant seeds. The method comprises the following steps: seed preprocessing: selecting plump seeds which are not damaged by diseases and insects, washing the seeds by tap water for 15-20min, and steeping the seeds in sterile water at 2-4 DEG C for 12-30h; then explant sterilizing: peeling off the flower glume of the preprocessed seeds, sterilizing by alcohol with the alcoholic strength of 75 percent for 25-40s, washing by sterile water for 3 times, sterilizing by 0.1 percent of mercuric chloride solution for 1-4min, washing by sterile water for 5 times again, then sucking the moisture on the surfaces of the seeds by sterile filter paper, and then transferring the seeds to a solid culture medium which is an MS;1 / 2MS culture medium; firstly, culturing in a light-proof enclosure, then adopting lighting culture when buds grow to be 1-2 cm in height, and then culturing sound seedlings, namely, the seedlings are transferred to a 1 / 2MS culture medium when the seedlings grows to be 8-10 cm in height so that the height of the seedlings is kept to be 10+ / -2 cm. The invention improves the germination ability of roegneria kamoji plants, increases the germination index from 14.48 to 42.06 and obtains uniform sterile seedlings, thereby satisfying the requirements of seed planting and genetic breeding experiments of roegneria kamoji plants.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for aseptic germination and cultivation of the seeds of the genus Amaranthus. Background technique [0002] The genus Roegneria was established by C. Koch in 1848 with R. caucasica C. Koch as the model species. It is the largest genus in the Triticea family. There are more than 130 species in the world. The genus Escherichia is a kind of plant with warm and cold characteristics. It is widely distributed and concentrated in Asia, Europe and North America. Already on the verge of the coast of the Arctic Ocean, the southern boundary extends to my country's Yunnan, Guangxi, and Taiwan regions and the Rocky Mountains in the western United States. There are more than 70 species of the genus Escherichia in my country, which are distributed from the Turpan Basin at -30 meters below sea level to the Himalayan ridge at an altitude of 5,500 meters (Journal of Northwest Bot...

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Problems solved by technology

[0003] Under the background of this research, the inventors found that the seeds of the genus Esperanza have small grains, less storage of nutrients, high dormancy, and inconsistent germination. There are many difficulties in obtaining neat and consistent aseptic seedlings.

At present, the aseptic seeding and germination technology has been successfully applied to rhizome plants such as flowers and medicinal materials. If the existing technology is applied to the plants of the genus Aphidia, there are many deficiencies: first, due to the dense structure of the seed coat of the genus A. One of the difficulties is disinfection. According to existing methods such as the patented orchid plant seed aseptic germination and plant cultivation method, patent number: 200510010764; soybean transgenic neutron disinfection treatment method, patent number: 02150780.5, use the above disinfection method None of the methods are suitable for the plants of the genus Escherichia genus. Either the seed disinfection is not thorough, a large amount of materials are contaminated, or the disinfectant treatment time is too long, which will cause poisonous effects on the seeds and cause deformed seedlings; secondly, it cannot solve the problem of inconsistent germination. It was found that many of the seeds inserted into the medium did not germinate, and uniform sterile seedlings could not be obtained.

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