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Method for extracting water soluble beta glucan from fruiting bodies of hericium erinaceus and product thereof

A technology of Hericium erinaceus and water solubility, which is applied in the field of extracting active ingredients of traditional Chinese medicine, can solve the problems of poor absorption, poor water solubility, and difficulty in complete extraction, and achieve the effect of enhancing water solubility and increasing yield

Active Publication Date: 2009-12-09
中科优术(黑龙江)科技产业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The large molecular weight part of Hericium erinaceus polysaccharide is poor in water solubility, and it is difficult to extract completely when using water as a solvent extraction in the separation and extraction. Even if it is extracted with lye, although the extraction yield is high, the polysaccharide product obtained after drying is still insoluble in water.
The human body does not absorb this macromolecular erinaceus polysaccharide well

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Weigh 1 kg of Hericium erinaceus fruiting body and pulverize it, pass through a 50-mesh sieve, add 5 liters of 0.1 mol / liter sodium hydroxide solution to the powder and extract for 1 hour, filter through a plate basket with a filter cloth of 0.5 micron diameter to obtain 7.2 liters of filtrate. Add 28.8 liters of absolute ethanol to the filtrate, let it stand for 2 hours, and filter it with a plate basket with a filter cloth of 0.5 microns to obtain a precipitate, which weighs a total of 150 grams after drying. Add 750 ml of water to the precipitate and stir at room temperature for 10 minutes, add 1 gram of fungal α-amylase solution (Shandong Jienuo Bioenzyme Co., Ltd.), adjust the pH value to 5.5 with 6 mol / L hydrochloric acid, and incubate at 50 degrees Celsius for 24 hours. Then filter with a plate basket of 0.5 micron pore size filter cloth, discard the filtrate, add 0.8 liter of 1.0 mol / liter sodium hydroxide solution to the residue, stir and extract for 2 hours, ad...

Embodiment 2

[0031]Weigh 1 kg of Hericium erinaceus fruiting body and pulverize it, pass through a 50-mesh sieve, add 10 liters of 1 mol / liter sodium hydroxide solution to the powder and extract for 5 hours, filter through a plate basket with a filter cloth of 5 microns in diameter to obtain 7.2 liters of filtrate. Add 28.8 liters of absolute ethanol to the filtrate, let it stand for 2 hours, and filter it with a plate basket of filter cloth with a pore size of 5 microns to obtain a precipitate, which weighs a total of 142 grams after drying. Add 1.4 liters of water to the precipitate and stir at room temperature for 10 minutes, add 0.6 g of α-amylase solution (purchased from Wuxi Chengxing Biotechnology Co., Ltd.), adjust the pH value to 5.8 with 2 mol / L acetic acid, and incubate at 50 degrees Celsius for 24 hours. Then filter with a plate basket of 5 micron aperture filter cloth, discard the filtrate, add 0.8 liters of 1.0 mol / liter of sodium hydroxide solution to the residue and stir for...

Embodiment 3

[0033] Take by weighing 1 kg of Hericium erinaceus fruiting body and pulverize it, cross a 50-mesh sieve, add 20 liters of sodium hydroxide solution of 0.5 mol / liter to the powder and extract for 10 hours, filter the residue with a plate basket of 2 micron aperture filter cloth, and obtain 7.2 liters of filtrate . Add 28.8 liters of dehydrated ethanol to the filtrate, and after standing for 2 hours, filter with a plate basket of filter cloth with a pore size of 2 microns to obtain a precipitate, which weighs a total of 143 grams after drying. Add 1 liter of water to the precipitate and stir at room temperature for 10 minutes, add 1 gram of fungal α-amylase solution (Shandong Jienuo Bioenzyme Co., Ltd.), adjust the pH value to 5.5, and incubate at 50 degrees Celsius for 24 hours. Then filter with a plate basket of 4 micron aperture filter cloth, discard the filtrate, add 0.6 mol / liter of sodium hydroxide solution and 0.8 liter of stirring to extract the residue for 2 hours, adj...

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Abstract

The invention discloses a method for extracting water soluble beta glucan from fruit bodies of hericium erinaceus, which comprises the steps of: adopting the fruit bodies of the hericium erinaceus as an extraction raw material; performing alkali extraction, ethanol deposition, alpha amylase hydrolysis and acid hydrolysis; and drying a precipitate after the ethanol deposition to obtain the water soluble beta glucan. The method also solves the problem of poor water solubility of beta glucan when alpha glucan is removed; polysaccharide obtained by the method is soluble beta glucan, the polysaccharide, namely the beta glucan of the fruit bodies of the hericium erinaceus has high content, and the average molecular weights are between 2,000 and 200, 000 daltons; and the water soluble beta glucan has excellent water solubility and can be used as a raw material for preparing medicaments, health care products and food.

Description

technical field [0001] The invention relates to a method for extracting water-soluble β-glucan from fruiting bodies of Hericium erinaceus and a product thereof, belonging to the field of extracting active components of traditional Chinese medicines. Background technique [0002] Hericium erinaceus, also known as Hericium erinaceus, Hericium erinaceus or hedgehog fungus, has a Latin name of Hericium erinaceus (Bull: Fr.) Pers. It belongs to the order Aphylloides, Hericiumaceae, and the genus Hericium erinaceus in taxonomy. It was milky white when it was just born, gradually turned yellowish, and turned yellowish brown after being collected and dried. Its shape resembles a monkey's head, so it is called Hericium erinaceus. Hericium erinaceus is a well-known food, which is said to be mountain delicacy Hericium erinaceus and seafood bird's nest. Hericium erinaceus is also a kind of precious medicinal material, which can treat diseases such as indigestion, gastric ulcer, duodena...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/02C12S3/02
Inventor 陈喜君王辉毛延卿金飞
Owner 中科优术(黑龙江)科技产业有限公司
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