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Method and device for preventing thallus in fermentation tank from adhering

A fermenter and bacteria technology, applied in the direction of bioreactor/fermenter combination, specific-purpose bioreactor/fermenter, biochemical cleaning device, etc., to achieve the effect of improving fermentation efficiency, convenient operation and simple device

Inactive Publication Date: 2009-11-25
QILU UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, as the surface of the fermentation liquid reaches a new height, some bacteria still continue to spread upwards from this height, and accumulate more and more on the new height. Therefore, this method cannot completely solve the problem of these microorganisms sticking to the wall. and the problem of spreading

Method used

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  • Method and device for preventing thallus in fermentation tank from adhering
  • Method and device for preventing thallus in fermentation tank from adhering
  • Method and device for preventing thallus in fermentation tank from adhering

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Effect test

Embodiment 1

[0030] according to Figure 1-3 As shown, the device of the present invention for implementing the method for preventing the adherence of bacterial cells in the fermentor includes a pipeline 1, a flow regulating valve 2 and a ring device 3, and the ring device 3 is fixed in the fermentor 9 through a positioning bracket 7. The annular device 3 is provided with injection holes 8.

[0031] In the process of fermenting Sorangium cellulosum ATCC 15384 to produce epothilone in a fermenter, the adhesion of the bacteria to the wall is serious. The method and device of the present invention relieve the adhesion of the bacteria. For the wall adhesion problem, the relevant parameters of the invention device are as follows:

[0032] The total volume of the fermentation tank V: 500 liters; liquid filling factor λ: 75%

[0033] The inner radius of the fermentation tank R: 290mm; the bending radius of the ring tube: 270mm

[0034] Number of injection holes opening N: 100; injection hole diameter ...

Embodiment 2

[0047] In the process of fermenting Aspergillus terreus ATCC 20542 to produce lovastatin, the adhesion of the bacteria to the wall is serious. The method and device of the present invention relieve the adhesion of the bacteria to the wall. For adhesion issues, the relevant parameters of the invention device are as follows:

[0048] The total volume of the fermentation tank V: 50 liters; liquid filling factor λ: 70%

[0049] The inner radius of the fermentation tank R: 150mm; the bending radius of the ring tube r: 130mm

[0050] Number of injection holes opening N: 60; injection hole diameter d: 1mm

[0051] Annular tube opening area A: 47.1mm 2 ; Annular tube diameter D: 8mm

[0052] Fluid type: sterile liquid; diameter of sterile air delivery pipe Φ: 15mm

[0053] Aseptic air pressure P: 0.2MPa; pressure in the fermentation tank p: 0.03MPa

[0054] The distance between the annular device and the fermentation liquid surface T: 30mm; the angle between the spray direction and the inne...

Embodiment 3

[0063] In the process of fermenting Sorangium cellulosum ATCC 15384 to produce epothilone in a fermenter, the adhesion of the bacteria to the wall is serious. The method and device of the present invention relieve the adhesion of the bacteria. For the wall adhesion problem, the relevant parameters of the invention device are as follows:

[0064] The total volume of the fermentation tank V: 500 liters; liquid filling factor λ: 75%

[0065] The inner radius of the fermentation tank R: 290mm; the bending radius of the ring tube: 270mm

[0066] Number of injection holes opening N: 40; injection hole diameter d: 0.2mm

[0067] Ring tube opening area A: 1.26mm 2 ; Annular tube diameter D: 6mm

[0068] Fluid type: sterile air; diameter of sterile air delivery pipe Φ: 20mm

[0069] Aseptic air pressure P: 0.15MPa; Pressure in the fermentation tank p: 0.03MPa

[0070] The distance between the ring device and the fermentation liquid surface T: 55mm; the angle between the spray direction and...

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Abstract

The invention discloses a method and a device for preventing thallus in a fermentation tank from adhering. The method uses sterile gas or sterile liquid, and blows or elutes the thallus to fermentation liquid against the direction of adherence extension of the thallus. The method is realized by arranging a set of annular porous device in a specific distance above the fermentation liquid level in the fermentation tank. The device has the technical advantages that the device is simple and convenient to operate and is not easy to be contaminated.

Description

Technical field [0001] The invention relates to a method and a device for preventing bacterial cells from sticking to a wall in a fermentation tank, and belongs to the technical field of fermentation engineering and equipment. Background technique [0002] When microorganisms such as myxobacteria and filamentous fungi are cultured in the fermentor, the bacteria easily adhere to the inner wall of the fermentor above the fermentation liquid level, and as the fermentation time increases, the bacteria gradually spread upward along the inner wall of the fermentor. The more and more bacteria adhere to the wall, this is a common phenomenon in the industrial production of microbial fermentation. [0003] This phenomenon has at least two disadvantages: one is that the adherent bacteria cannot return to the liquid, resulting in a decrease in the number of bacteria cells in the fermentation broth, and the reduction of biomass, resulting in low fermentation efficiency; second, adherence Bact...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14C12M1/00
CPCC12M39/00
Inventor 刘新利胡玮崔灵芝
Owner QILU UNIV OF TECH
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