Immunoliposome based on endostatin gene, preparation method and application thereof

Abstract

The invention discloses immunoliposome based on endostatin genes, a preparation method and the application thereof. The immunoliposome comprises Vasohibin gene recombination eukaryotic expression vector, liposome and anti-eighth factor-associated antigen monoclonal antibody; wherein the liposome internally wraps up the Vasohibin gene recombination eukaryotic expression vector; the surface of the liposome is connected with the anti-eighth factor-associated antigen monoclonal antibody; the preparation method thereof comprises three steps of: the construction of the Vasohibin gene recombination eukaryotic expression vector, the preparation of the liposome wrapping up the Vasohibin gene recombination eukaryotic expression vector, and the connection of the liposome wrapping up the Vasohibin gene recombination eukaryotic expression vector and the anti-eighth factor-associated antigen monoclonal antibody. The immunoliposome can particularly act on vascular endothelial cells of the lung to inhibit the vasculogenesis and pulmonary fibrosis formation, has strong targeting effect, little side effect and can be used for preparing anti-fibrosis drugs.

Description

technical field [0001] The invention relates to the field of genetic engineering, in particular to the immunoliposome based on the endostatin (Vasohibin) gene, and also relates to the preparation method and application of the immunoliposome. Background technique [0002] Pulmonary fibrosis (PF) is a large group of diseases characterized by diffuse exudation, infiltration and fibrosis of the pulmonary interstitium. Its pathogenesis is still unclear, there is a lack of specific therapeutic drugs in clinical practice, and the prognosis is extremely poor. Therefore, the research on the pathogenesis and prevention of pulmonary fibrosis has important clinical significance. [0003] It is traditionally believed that chronic inflammation plays an important role in pulmonary fibrosis, however, the measures targeting single or multiple cytokines to inhibit the inflammatory response to alleviate pulmonary fibrosis have been proved to be ineffective. In recent years, the role of angio...

AI Technical Summary

Problems solved by technology

[0003] It is traditionally believed that chronic inflammation plays an important role in pulmonary fibrosis, however, the measures targeting single or multiple cytokines to inhibit the inflammatory response to alleviate pulmonary fibrosis have been shown to be ineffective

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