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Method for determining content of compound Caoshanhu lozenge

A technology of grass coral buccal tablets and determination methods, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of no reports and can not reflect the quality of buccal tablets well, and achieve enhanced controllability and enhanced Effects of plausibility and reliability

Active Publication Date: 2009-11-11
JIANGZHONG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, in the 2005 Pharmacopoeia Standards, the quality control method adopted for the Compound Caoshanhu Buccal Tablets is to identify the menthol and sargassum extract in the item [identification] except that the menthol and sargassum extract are identified in the item [identification], and only the determination of The content of isoferidin in it has been checked, although it has been reported in the literature that isoferidin has a certain anti-tumor effect and its content in the buccal tablet is relatively high, but there is no report that it has the functions of antibacterial, anti-inflammatory, etc. and compound grass coral buccal tablets. Indication-related pharmacological activity
Therefore, only Isoferidin is used as the containment index in the quality control method of the buccal tablet, and the result of the containment test cannot reflect the quality of the buccal tablet well.

Method used

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  • Method for determining content of compound Caoshanhu lozenge
  • Method for determining content of compound Caoshanhu lozenge
  • Method for determining content of compound Caoshanhu lozenge

Examples

Experimental program
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Effect test

Embodiment 1

[0045] A method for determining the content of compound grass coral buccal tablets, comprising using thin-layer chromatography to identify menthol and sargassum extract, and using high-performance liquid chromatography to simultaneously determine isoquinone in it under the chromatographic conditions of isocratic elution. The content of two index components, pyridine and rosmarinic acid. Specific steps include:

[0046] 【Content Determination】

[0047] (1) Chromatographic conditions and system suitability test Octadecylsilane bonded silica gel was used as filler; acetonitrile-0.1% phosphoric acid solution (20:80) was used as mobile phase; detection wavelength was 342nm. The number of theoretical plates should not be less than 1200 based on the peak of isoferidinium.

[0048] (2) Preparation of reference solution: Take appropriate amount of isoferidin reference substance and rosmarinic acid reference substance, weigh them accurately, add methanol to make solutions containing 8...

Embodiment 2

[0053] A method for determining the content of compound grass coral buccal tablets, comprising using thin-layer chromatography to identify menthol and sargassum extract, and using high-performance liquid chromatography to simultaneously determine isoquinone in it under the chromatographic conditions of isocratic elution. The content of two index components, pyridine and rosmarinic acid. Specific steps include:

[0054] 【Content Determination】

[0055] (1) Chromatographic conditions and system suitability test Octadecylsilane bonded silica gel was used as filler; acetonitrile-0.1% phosphoric acid solution (30:70) was used as mobile phase; detection wavelength was 270nm. The number of theoretical plates should not be less than 1200 based on the peak of isoferidinium.

[0056] (2) Preparation of reference substance solution Take appropriate amount of isoferidin reference substance and rosmarinic acid reference substance, weigh them accurately, add methanol to make a solution co...

Embodiment 3

[0061] A method for determining the content of compound grass coral buccal tablets, using high performance liquid chromatography to simultaneously determine the content of four index components, protocatechuic acid, caffeic acid, isoferidin, and rosmarinic acid, under gradient elution chromatographic conditions content, the specific steps include:

[0062] 【Content Determination】

[0063] (1) chromatographic conditions and system suitability test use octadecylsilane bonded silica gel as filler; mobile phase A is acetonitrile, mobile phase B is 0.1% phosphoric acid (containing 2.5% acetonitrile); flow rate is 1.0ml per minute, Linear gradient elution; column temperature is 38°C; detection wavelength is 294nm. The number of theoretical plates calculated based on the peak of isoferidin should not be less than 1200, and the resolution of the peaks of protocatechuic acid, caffeic acid, isoferidin and rosmarinic acid should not be less than 2.0. Mobile phase gradient change Mobile...

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Abstract

The invention relates to a method for determining the content of compound Caoshanhu lozenge prepared from glabrous sarcandra herb extractum, menthol and dementholised peppermint oil. The thin-layer chromatography is used for distinguishing the menthol and the glabrous sarcandra herb extractum in the lozenge. The method is characterized in that the high performance liquid chromatography is used forsimultaneously determining the content of two marker components, including isofraxidin and rosmarinic acid in the lozenge under the chromatographic condition of isocratic elution; or the high perform ance liquid chromatography is used for simultaneously testing the content of four marker components, including protocatechuic acid, caffeic acid, the isofraxidin and the rosmarinic acid in the lozengeunder the chromatographic condition of gradient elution.

Description

technical field [0001] The invention relates to a method for determining the content of a compound traditional Chinese medicine preparation, in particular to a method for determining the content of a compound Chinese medicine grass coral buccal tablet. Background technique [0002] The compound grass coral buccal tablets recorded in "Pharmacopia of the People's Republic of China" (2005 edition one) is a traditional Chinese medicine compound preparation prepared from sargassum extract, menthol, and peppermint oil. [Preparation method] the above three flavors, Zhongjiefeng extract is taken from Zhongjiefeng, add water and decoct twice, the first time is 2 hours, the second time is 1.5 hours, combine the decoction, filter, the filtrate is concentrated to a relative density of 1.15 (80°C), add ethanol When the alcohol content reaches 65%, let stand for 24 hours, filter, recover ethanol from the filtrate under reduced pressure, and concentrate it into a clear paste with a relativ...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/36G01N30/90
Inventor 郭勇金李诒光尧梅香罗跃华卢建中
Owner JIANGZHONG PHARMA
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