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Cold water coagulable culture medium coagulator and preparation method thereof

A technology of coagulant and culture medium, applied in the field of culture medium coagulant and its preparation, can solve the problems of colony pollution, easy crystallization or cracking, high price, etc., achieve good glue strength and water retention, not easy to be stained, not easy to The effect of decomposition

Active Publication Date: 2009-11-04
GUANGDONG INST OF MICROORGANISM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, agar is the most widely used coagulant, but there are still problems: 1) excessive mining leads to high price, 2) gel moisture is easy to volatilize, 3) condensed water accumulates on the wall of the petri dish and easily causes contamination between colonies, 4) freezing Easy to crystallize or crack, which is not conducive to the passage of strains and long-term storage
In addition, because agar cannot be solidified in cold water, when preparing microbial culture medium, it needs to be heated and cooled before being poured into the plate, which greatly affects the detection efficiency

Method used

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  • Cold water coagulable culture medium coagulator and preparation method thereof
  • Cold water coagulable culture medium coagulator and preparation method thereof
  • Cold water coagulable culture medium coagulator and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0029] The cold water coagulable culture medium coagulant described in this embodiment is prepared from the following xanthan gum, guar gum, sodium polyacrylate and locust bean gum proportioning, and its preparation method steps are as follows:

[0030] (1) pulverize xanthan gum, guar gum, sodium polyacrylate and locust bean gum respectively, and cross a 160-mesh sieve;

[0031] (2) mix the xanthan gum and guar gum after sieving with a mass ratio of 1: 1.5;

[0032] (3) Sodium polyacrylate and locust bean gum after sieving are mixed evenly with a mass ratio of 1:9;

[0033] (4) Mix the mixture of step (2) and (3) uniformly in a mass ratio of 8:1 to obtain a cold water condensable culture medium coagulant;

[0034] (5) Take 0.5g of powder, add 100ml of deionized water, and sterilize in a Erlenmeyer flask at 110°C for 10min;

[0035] (6) Take 10ml, add dropwise to a plate with a diameter of 7cm, and dry at 70°C for 5 hours to make a rapid test plate for use;

[0036] (7) The ...

Embodiment 2

[0038] The cold water coagulable culture medium coagulant described in this embodiment is prepared from the following xanthan gum, guar gum, sodium polyacrylate and locust bean gum proportioning, and its preparation method steps are as follows:

[0039] (1) Pulverize xanthan gum and guar gum respectively, cross 160 mesh sieves;

[0040] (2) mix the sieved xanthan gum and guar gum with a mass ratio of 1:1;

[0041] (3) Sodium polyacrylate and locust bean gum after sieving are mixed evenly with a mass ratio of 1:8;

[0042] (4) Mix the mixture of step (2) and (3) uniformly in a mass ratio of 9:1 to obtain a cold water condensable culture medium coagulant;

[0043] (5) After drying at 70°C for 6 hours, take 0.2g of the plastic film stuck on the upper layer of the rapid test piece;

[0044] (6) Assemble the lower layer of film with the chromogenic medium, the bottom plate and the plastic film with the coagulant to make a quick test piece for future use.

[0045] (7) The water a...

Embodiment 3

[0047] The cold water coagulable culture medium coagulant described in this embodiment is prepared from the following xanthan gum, guar gum, sodium polyacrylate and locust bean gum proportioning, and its preparation method steps are as follows:

[0048] (1) pulverize xanthan gum, guar gum, sodium polyacrylate and locust bean gum respectively, and cross a 160-mesh sieve;

[0049] (2) mix the xanthan gum and guar gum after sieving with a mass ratio of 1: 1.2;

[0050] (3) Sodium polyacrylate and locust bean gum after sieving are mixed evenly with a mass ratio of 1:8;

[0051] (4) Mix the mixture of step (2) and (3) uniformly in a mass ratio of 8:1 to obtain a cold water condensable culture medium coagulant;

[0052] (5) Take 0.8g of powder, add 100ml of deionized water, and sterilize in a Erlenmeyer flask at 110°C for 10min;

[0053] (6) Take 10ml, add dropwise to a plate with a diameter of 7cm, and dry at 70°C for 5 hours to make a rapid test plate for use;

[0054] (7) The ...

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Abstract

The invention discloses a cold water coagulable culture medium coagulator and a preparation method thereof. The culture medium coagulator is mainly prepared from the following raw materials by weight portion: 1 portion of xanthan gum and 0.5 to 1.5 portions of guar gum which are mixed together; 1 portion of polyacrylate sodium and 7 to 10 portions of locust bean gum which are mixed together, wherein the mass ratio of the mixture of the xanthan gum and the guar gum to the mixture of the polyacrylate sodium and the locust bean gum is 7-9:1. The cold water coagulable culture medium coagulator can be dissolved and coagulated in cold water, has good water absorption, short reaction time and high gel strength and viscosity, is suitable for the growth of most microbes, and can form gel. The gel can be used as the coagulator for a microbe culture medium, thus the cold water coagulable culture medium coagulator avoids the defects that the prior agar culture medium needs heating and consumes more time for cooling, and an agar plate is easy to dehydrate and check.

Description

technical field [0001] The invention relates to a cold-water condensable medium coagulant for microbial growth and cultivation and a preparation method thereof, belonging to the field of polymer materials. Background technique [0002] Since the German bacteriologist Koch first used gelatin as a solid medium coagulant for bacterial streaking, the coagulant has played an important role in bacterial culture, isolation, and identification. Subsequently, each hydrophilic coagulant was discovered one after another, and after being dissolved in water, it swelled to form a viscous solution or gel, and was used in microbial culture medium. At present, agar is the most widely used coagulant, but there are still problems: 1) excessive mining leads to high price, 2) gel water is easy to volatilize, 3) condensed water accumulates on the wall of the petri dish and easily causes contamination between colonies, 4) freezing It is easy to crystallize or crack, which is not conducive to the ...

Claims

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Application Information

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IPC IPC(8): C12N1/00C12N1/20C12Q1/06C12Q1/10C12Q1/14C08L5/00C12R1/42C12R1/19C12R1/445C12R1/125C12R1/385
Inventor 吴清平吴许文张淑红张菊梅
Owner GUANGDONG INST OF MICROORGANISM
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