Method for mutagenesis breeding of high temperature resistant asparagus excellent variety series

A technology of Astragalus and high temperature resistance, which is applied in the field of bioengineering, can solve the problems of slow growth rate, low glue content, poor high temperature resistance performance, etc., and achieves fast growth rate, high glue content and improved glue content Effect

Inactive Publication Date: 2009-10-28
OCEAN UNIV OF CHINA
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AI Technical Summary

Problems solved by technology

[0008] The invention provides a method for mutagenesis and breeding of excellent strains of high-temperature-resistant asparagus, which can solve the problems of poor high-temperature resistance, slow growth rate and low glue content of existing wild-type asparagus

Method used

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  • Method for mutagenesis breeding of high temperature resistant asparagus excellent variety series
  • Method for mutagenesis breeding of high temperature resistant asparagus excellent variety series
  • Method for mutagenesis breeding of high temperature resistant asparagus excellent variety series

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Effect test

Embodiment 1

[0061] 1) The method for mutagenesis and breeding of asparagus

[0062] Get long 20-50mm stem tip as mutagenesis material, then place in the MNNG treatment solution of 30mg / L and carry out mutagenesis, the treatment time is respectively 0, 10, 20, 30, 40 and 50min, the material after treatment is in f / 2 culture medium in the dark for 24 hours and then moved to light culture.

[0063] The results showed that the shoot tip still had normal germination and growth ability after being mutated by MNNG. But its survival rate has a certain relationship with the mutagenesis time. Depend on figure 1 It can be seen that: MNNG has obvious inhibitory effect on wild-type asparagus, the longer the mutagenesis time, the lower the shoot tip survival rate, and when the mutagenesis time reaches more than 30min, 50% of the shoot tips cannot survive. On the 3rd day after treatment, the survival rate of the 10min treatment group was 90%, while that of the 50min treatment group was only 75%. On...

Embodiment 2

[0068] Determination of High Temperature Resistance of Two Strains

[0069] Select 3 strains of wild-type asparagus and 07-2, respectively, and place them in a 100ml Erlenmeyer flask with 80ml of culture medium, and culture them in a light constant temperature incubator at 40°C to observe the shape and pigment changes of the algae.

[0070] The same high temperature stress (40℃) caused different damage and rot to 07-2 and wild-type asparagus. The time and degree of disease and rot in 07-2 were significantly later than that of wild-type asparagus. After 12 hours of stress, there was no obvious change in the morphology of the two, and the branches remained intact without disease and rot (fading and rotten shoots). At 24 hours, the branches of wild-type asparagus began to fade and turn white, and only a small amount of branches turned white and died in 07-2. At 48 hours, the fading area of ​​wild-type asparagus was expanded and the main branches were pink, while the main branche...

Embodiment 3

[0072] Comparison of line growth speed of two strains

[0073] Wild-type asparagus and 07-2 respectively select 50 young algae tips with a length of 10mm, put them in a 100ml Erlenmeyer flask and add 80ml of medium, measure the length after one week of cultivation, if there are branches, add all branches The total length was continuously measured for four weeks, and the average linear growth velocity was calculated. Medium was changed weekly.

[0074] For the growth status of 07-2 and wild type asparagus, see Figure 4 , showing a significant difference. The average linear growth rate of 07-2 was 0.90mm / d; that of wild type asparagus was 0.77mm / d, and there was a significant difference between the two (p0.05), but at 28 days there was a significant difference (p<0.05), and the average length of wild-type asparagus The average length of the 07-2 algae body is 45.90mm, showing a relatively obvious growth advantage.

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Abstract

The invention provides a method for mutagenesis breeding of a high temperature resistant asparagus excellent variety series, which can solve the problems that in the prior art a wild type asparagus has poor high temperature resistance, low growth rate and low gel content. The method for the mutagenesis breeding comprises the following steps: a, MNNG mutagenesis: soaking trichomes of the asparagus in an MNNG treating fluid to perform the mutagenesis; and b, HYP resistance series selection: putting the mutagenized trichomes of the asparagus into an f / 2 culture medium containing HYP to perform high-temperature cultivation, and screening out the required high temperature resistant strains. The asparagus variety obtained by the method not only is high temperature resistant, but also has high growth rate and higher gel content, thus the method provides a new variety series for cultivating the asparagus, and is significant for promoting the development of the agar manufacturing industry.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a method for mutagenesis and breeding of high-temperature-resistant asparagus excellent strains. Background technique [0002] my country's algae cultivation has a history of more than 60 years, and its output accounts for more than 70% of the world. The cultivated species mainly include kelp, seaweed, wakame and so on. Algal cultivation creates significant economic, social and ecological benefits. However, compared with marine economic animal farming, the industry and product structure update develops slowly. [0003] Agar gum is an indispensable and important raw material in industries such as food, chemical industry, medicine and bioengineering, and has a huge market demand. At the end of the 1990s, the world's annual output of agar was about 15,000 tons (Ji Minghou: "Seaweed Chemistry"), and it has exceeded 20,000 tons at present. But the cultivation of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/04A01H1/06
Inventor 张学成徐涤孟琳臧晓南
Owner OCEAN UNIV OF CHINA
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