Manufacturing method of paraffin tissue chip
A production method and tissue chip technology, which are applied in the preparation of test samples, material inspection products, biological testing, etc., can solve the problems of limited tissue thickness, small tissue, and difficulty in providing information on tissue structure, and achieve high output and high quality materials. various effects
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Embodiment 1
[0020] Paraffin tissue chips were prepared by the following method:
[0021] Step 1: Take the required tissues when collecting pathological materials, number them, cut them into a size of 2mm×2mm×10mm, follow the routine tissue processing procedures of the pathology department, and go to the step of soaking them in paraffin; put them on the ironing table at 60°C The paraffin on the surface is melted to obtain the tissue to be embedded;
[0022] Step 2: Take another paraffin and put it into a paraffin pot, and heat it to 60°C with an electric furnace to melt the paraffin;
[0023] Step 3: Put 2 vertically bent copper strips on a thick glass plate, put together a square wax mold with a length of 70mm, a width of 40mm, a height of 20mm, and a thickness of 10mm on a glass plate, with a thickness of (copper The height of the strip), use tweezers to take the first tissue to be embedded, quickly immerse it once in melted paraffin, stick the flat surface to the glass, rely on the sti...
Embodiment 2
[0025] Paraffin tissue chips were prepared by the following method:
[0026] Step 1: Number and position the required tissue on the wax block, cut the tissue into a square block with a length of 5mm, a width of 1mm, and a thickness of 1mm; put it on the ironing table, and melt the paraffin on it at 65°C Obtain the tissue to be embedded;
[0027] Step 2: Take another paraffin and put it into a paraffin pot, and heat it to 70°C with an electric furnace to melt the paraffin;
[0028] Step 3: Put 2 vertically bent copper strips on a thick glass plate, put together a square wax mold with a length of 70mm, a width of 40mm, a height of 20mm, and a thickness of 20mm on a glass plate, with a thickness of (copper The height of the strip), use tweezers to take the first piece of tissue to be embedded, quickly immerse it once in melted paraffin, stick the flat surface to the glass, rely on the stickiness of the paraffin to stand the tissue on the glass, the first piece of tissue has The...
Embodiment 3
[0030] Paraffin tissue chips were prepared by the following method:
[0031] Step 1: Number and position the required tissue on the wax block, cut the tissue into a cylinder with a length of 10mm and a radius of 1mm; put it on the ironing table, and melt the paraffin on it at 65°C to obtain the tissue to be embedded organization;
[0032] Step 2: Take another paraffin and put it into a paraffin pot, and heat it to 70°C with an electric furnace to melt the paraffin;
[0033]Step 3: Put 2 vertically bent copper strips on a thick glass plate, put together a square wax mold with a length of 70mm, a width of 40mm, a height of 20mm, and a thickness of 20mm on a glass plate, with a thickness of (copper The height of the strip), use tweezers to take the first tissue to be embedded, quickly immerse it once in melted paraffin, stick the flat surface to the glass, rely on the stickiness of the paraffin to stand the tissue on the glass, the first tissue has two The side is close to the ...
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