Method for extracting highland barley Beta-dextran and dietary fiber by combined-enzyme method
A technology of dietary fiber and glucan, applied in food preparation, food science, application and other directions, can solve the problem that beta-glucan has not been developed and utilized, and achieves increased economic added value, broad application prospects, and increased economic income. Effect
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[0025] The highland barley bran is first processed by a bran beating machine, then sieved to remove the sticky flour in the bran, and then crushed through a 40-mesh sieve. Enzymatic hydrolysis: After pulverizing the bran, the ratio of material to water is 1:6, adjust the pH to 5 with dilute hydrochloric acid, add cell wall decomposing enzyme (xylanase, cellulase or pentosanase, 40u / 100mL), at 45°C Enzymolyze for 1 hour, centrifuge, and then use an extruder to process the bran. On the one hand, the enzyme is eliminated, and on the other hand, the structure of the bran is further loosened, which is convenient for subsequent extraction and separation. Then, it is crushed through a 40-mesh sieve, and 30g of crushed bran is weighed. Put the skin sample into a reactor equipped with a stirring device, add 300mL of water, 120u of cellulase, stir in a water bath at 45°C for 1h, then adjust the pH to 10 with anhydrous sodium carbonate, and stir in a water bath at 45°C for 1h; take out th...
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