Powder for chicken sperm dilution and preservation and preparation method and application thereof

A semen and diluent technology, applied in the field of artificial insemination of poultry, can solve the problems of long storage time, hectic transportation of chicken semen, and unsolved problems of chicken semen preservation, achieve low cost, enrich research results, and improve immediate insemination busy effect

Inactive Publication Date: 2009-09-30
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the patent does not solve the technical problem of chicken semen preservation for a longer period of time, and preservation for 7 hours cannot fundamentally solve the hectic work of transportation and insemination of chicken semen, and requires longer preservation time in actual production and application

Method used

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  • Powder for chicken sperm dilution and preservation and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1 Prepare raw materials according to the dilution powder formula given in Table 1:

[0035] Table 1 Dilution powder formula

[0036] Reagent content

[0037] glucose 0.2400g Sodium citrate 0.0250g sodium glutamate 0.4005g K 2 HPO 4 .3H 2 o 0.62055g K 2 HPO 4 0.0325g Sodium acetate trihydrate 0.2050g NaCl 0.0900g Tris 0.0415g penicillin 0.0100g

[0038] Various chemicals (analytically pure) in the purchased formula, large particles or components that have been agglomerated need to be crushed and sieved with an 80-mesh sieve to form a powder.

[0039] Accurately weigh various ingredients, mix according to the ratio in the formula, and fully stir, then pack into bags, vacuumize and seal to obtain 1 pack of chicken semen dilution powder.

[0040] The above process generally requires the temperature to be controlled at 18-26°C and the relative humidity to be controlled at 45-65...

Embodiment 2

[0045] Embodiment 2 Prepare raw materials according to the dilution powder formula given in Table 2:

[0046] Table 2 Diluent powder formula

[0047] Reagent content glucose 0.2000g Sodium citrate 0.0150g sodium glutamate 0.4500g K 2 HPO 4 .3H 2 o 0.5500g K 2 HPO 4 0.0500g Sodium acetate trihydrate 0.3000g NaCl 0.0500g Tris 0.0900g Chloramphenicol 0.0100g

[0048] Other operations are the same as in Example 1. Add 60ml of distilled water to dissolve completely and adjust the pH value to 8.0. Dilute the semen with the diluent at a ratio of 1:2 and then directly inseminate it, or store it at 4°C for later use.

Embodiment 3

[0049] Embodiment 3 Prepare raw materials according to the dilution powder formula given in Table 3:

[0050] Table 3 Dilution powder formula

[0051] Reagent content glucose 0.3000g Sodium citrate 0.0270g sodium glutamate 0.3500g K 2 HPO 4 .3H 2 o 0.7500g K 2 HPO 4 0.0100g Sodium acetate trihydrate 0.3000g NaCl 0.1500g Tris 0.0100g streptomycin 0.0100g

[0052] Various chemicals (analytically pure) in the purchased formula, large particles or components that have been agglomerated need to be crushed and sieved with an 80-mesh sieve to form a powder. Other operations are the same as in Example 1. The diluted semen can be directly inseminated or stored at 4°C for later use.

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Abstract

The invention discloses a powder for chicken sperm dilution and preservation and the preparation method and the application thereof. The powder for chicken sperm dilution and preservation comprises the following components according to the weight portion: 20-30 of glucose, 1.5-2.7 of sodium citrate, 35-45 of sodium glutamate, 55-75 of K2HPO4.3H2O, 1-5 of K2HPO4, 15-30 of trihydrate sodium acetate, 5-15 of NaCl and 1-9 of Tris; diluent is obtained after the powder is dissolved by distilled water and is adjusted in the pH value, and disinfectant can be added to the diluent. The powder for chicken sperm dilution and preservation can be applied to low-temperature long-term chicken sperm preservation or chicken sperm dilution. The invention has simple prescription component, low cost, long chicken sperm preservation time, good chicken sperm preservation effect, and high fertilization rate of hatching eggs after the chicken sperm is diluted and delivered.

Description

technical field [0001] The invention belongs to the technical field of artificial insemination of poultry, and in particular relates to a chicken semen dilution and preservation powder, a preparation method thereof and an application in low-temperature long-term preservation and dilution of chicken semen. Background technique [0002] With the rapid development of modern poultry industry, the scale and intensification of poultry farming in China are getting higher and higher, and poultry artificial insemination technology has also been widely used. [0003] Chicken artificial insemination technology not only significantly improves the fertilization rate of hen eggs, but also greatly reduces production costs. Due to the large number of roosters, the high frequency of semen collection, the small amount of semen collected, and the high density of sperm, it is required that the semen of roosters must be diluted. At the same time, dilution of chicken semen can increase the volum...

Claims

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Application Information

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IPC IPC(8): A01N1/02
Inventor 张守全胡艳娟
Owner SOUTH CHINA AGRI UNIV
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