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Hepatitis B virus pre S1 antigen chemiluminscence immunoassay kit and preparation method thereof

A technology of hepatitis B virus and reagent kit, which is applied in the field of immunoanalysis medicine, can solve problems such as the inability to widely apply clinical diagnosis and scientific research work, and limit popularization and use, and achieve industrialization and quality control, no cross-reaction, and clinical The effect of improving the matching rate

Inactive Publication Date: 2009-09-23
北京科美东雅生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In addition, the chemiluminescence immunoassay kit in the prior art is a closed fully automatic chemiluminescence measurement system, which requires an expensive fully automatic chemiluminescence measuring instrument, thus limiting its popularization and use, and cannot be widely used in clinical diagnosis and scientific research.

Method used

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  • Hepatitis B virus pre S1 antigen chemiluminscence immunoassay kit and preparation method thereof
  • Hepatitis B virus pre S1 antigen chemiluminscence immunoassay kit and preparation method thereof

Examples

Experimental program
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Embodiment 1

[0034] Example 1 Preparation of Hepatitis B Virus Pre-S1 Antigen Chemiluminescence Immunoassay Assay Kit of the present invention

[0035] 1. Preparation of acridinium ester-labeled antibody and biotin-conjugated antibody

[0036] 1. Acridine ester labeling method

[0037] (1) Add 500 μl of 1 mg / ml antibody to be labeled (pH7.20.050M PBS) into a brown glass bottle;

[0038] (2) Add 10 μl of acridinium ester (5 mM DMF solution) into the above-mentioned brown glass bottle, and react with the antibody to be labeled at room temperature in the dark for 30 minutes;

[0039] (3) Add 100 μl of 30 mg / ml lysine solution to terminate the reaction;

[0040] (4) ammonium sulfate precipitation, sephadex column separation, and purification of the acridinium ester-labeled antibody;

[0041] (5) Add the labeled antibody to 1% BSA, 50% glycerol and freeze it.

[0042] 2. Preparation of biotin-conjugated hepatitis B virus pre-S1 monoclonal antibody

[0043] (1) Dissolve biotin (BNHS) in N, N-...

Embodiment 2

[0095] Example 2 Preparation of the hepatitis B virus pre-S1 chemiluminescence immunoassay assay kit of the present invention

[0096] Except for using plastic beads as the solid-phase carrier, the rest were prepared in the same manner as in Example 1 to prepare the hepatitis B virus pre-S1 chemiluminescent immunoassay assay kit.

Embodiment 3

[0097] Example 3 Preparation of Hepatitis B Virus Pre-S1 Chemiluminescence Immunoassay Assay Kit of the present invention

[0098] Except that the magnetic particles were used as the carrier and the magnetic particle-immobilized avidin was prepared by the glutaraldehyde coupling method, the remaining hepatitis B virus pre-S1 chemiluminescent immunoassay assay kits were prepared in the same manner as in Example 1.

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Abstract

The invention provides a hepatitis B virus pre S1 antigen chemiluminscence immunoassay kit and a preparation method thereof, belonging to the medical field of immune analysis. The kit comprises: (1) hepatitis B virus pre S1 antigen calibrator; (2) carrier of avidin; (3) hepatitis B virus pre S1 monoclonal antibody of biotinylation; (4) anti-HBs monoclonal antibody marked with acridinium ester; (5) chemiluminescence initiating agent; and (6) concentrated cleaning solution. The method for preparing the kit further comprises the following steps: (1) the calibrator is prepared by using the hepatitis B virus pre S1 pure product; (2) the carrier is coated with the avidin; (3) the biotinylation is performed to the hepatitis B virus pre S1 monoclonal antibody; (4) the anti-HBs monoclonal antibody is marked with the acridinium ester; (5) the chemiluminescence initiating agent is prepared; (6) the concentrated cleaning solution is prepared; (7) the calibrator, the biotinylated antibody, the acridinium ester marker, the chemiluminescence initiating agent and the concentrated cleaning solution are subpacked; and (8) the finished product is assembled. The kit is simple, convenient, rapid, sensitive, stable, and the like.

Description

technical field [0001] The invention relates to the field of immunoanalysis medicine, specifically, the invention provides a hepatitis B virus pre-S1 antigen assay kit and a preparation method thereof, which combine biotin-avidin indirect coating technology and chemiluminescence immunoassay technology . Background technique [0002] Hepatitis B virus belongs to the family of hepatotropic viruses, which can cause acute hepatitis, chronic hepatitis and liver cirrhosis, and is closely related to the occurrence of liver cancer. China is a high-endemic area of ​​hepatitis B, but there is no effective antiviral method to treat chronic hepatitis B virus infection. Therefore, virus prevention and detection are important means to avoid infection. It provides important information for the development and prognosis of the disease. [0003] HBV surface antigen is the outer membrane protein of HBV, encoded by the S region of HBV genome. After HBV surface antigen is expressed by three ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/576G01N33/569G01N33/543G01N21/76
Inventor 彭京胜应希堂胡国茂唐宝军于尚永
Owner 北京科美东雅生物技术有限公司
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