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Method for screening degradation bacteria strains by taking high-efficiency cyhalothrin as substrate

A technology of lambda-cyhalothrin and screening methods, which is applied in biochemical equipment and methods, measurement/testing of microorganisms, separation of microorganisms, etc., to achieve good application prospects, simple and feasible screening methods, and high feasibility

Active Publication Date: 2009-09-23
辽宁中科生物工程股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Related degrading bacteria and enzyme preparations have not been reported

Method used

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  • Method for screening degradation bacteria strains by taking high-efficiency cyhalothrin as substrate
  • Method for screening degradation bacteria strains by taking high-efficiency cyhalothrin as substrate
  • Method for screening degradation bacteria strains by taking high-efficiency cyhalothrin as substrate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Serratia marcescens can degrade lambda-cyhalothrin.

[0026] Screening of lambda-cyhalothrin-degrading bacteria:

[0027] 1) Bacteria sample collection: collected from the soil in the pesticide factory of Shahe Town, Sujiatun District, Shenyang City, Liaoning Province.

[0028] 2) Enrichment and acclimatization: 10 g of bacterial samples were inoculated into 100 ml enrichment medium containing lambda-cyhalothrin for transfer culture, and the culture conditions for each transfer were 30°C, 180r / min shaker culture for 7 days, A total of 4 transfers were made, and the transfer amount was calculated by volume percentage. 10% of each culture solution was inoculated in 100ml enrichment medium containing lambda-cyhalothrin, which was gradually increased. The / L gradually increased, and the lambda-cyhalothrin was 25, 50, 75, and 100 mg / L during the 4 transfers, and the bacteria samples were added to the enrichment medium for the first time and 15 glass beads were added at the ...

Embodiment 2

[0035] Identification of Degradative Ability of Strains

[0036] 1. UV spectrophotometry

[0037] 1) Standard curve drawing: Dissolve the efficient-cyhalothrin standard substance in n-hexane, prepare standard solutions with different concentrations (see Table 1), measure the absorbance at 278nm, and draw the standard curve as y=0.0041x+0.0012 R 2 =0.9999 (see figure 1 ).

[0038] Table 1 standard curve measured value

[0039] Concentration mg / l

Absorbance at 278nm

0.002202

0

0.110008

0.0066

0.05504

0.0023

0.2752

0.00325

1.376

0.0072

6.88

0.0265

34.4

0.1369

172

0.70115

[0040] 2) Identification of degradation activity: The 16 strains of degrading bacteria that were screened out using lambda-cyhalothrin as a substrate were shaken and cultivated in ordinary medium until OD600=1.0, and then 10% of the bacterial liquid was taken by volume p...

Embodiment 3

[0053] The difference from Example 1 is:

[0054] 1) Enrichment and acclimatization: Inoculate 5 g of bacterial samples into 80 ml enrichment medium containing lambda-cyhalothrin for transfer culture, and the conditions for each transfer culture are 25°C, 130r / min shaker culture for 5 days, A total of 7 transfers were made, and the transfer amount was calculated by volume percentage. 5% of each culture solution was inoculated in 80ml enrichment medium containing lambda-cyhalothrin, which was gradually increased, and was set aside; / L gradually increased, and lambda-cyhalothrin was 25, 55, 85, 115, 145, 175, and 205 mg / L during the 7 transfers, and the bacteria samples were added to the enrichment medium for the first time and 10 glass beads were added at the same time ; The enrichment medium is: peptone 8g, NaCl 0.8g, KH 2 PO 4 0.8g, glucose 1.0g, H 2 O 1000ml, pH=6.8.

[0055] 2) Screening: Inoculate the enriched culture solution in step 1) into 60 ml of basal medium con...

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Abstract

The invention belongs to the field of biodegrading pesticide residue, and in particular relates to a method for screening degradation bacteria strains by taking high-efficiency cyhalothrin as a substrate. The screening method is a method for obtaining degradation bacteria which grow by taking the high-efficiency cyhalothrin as a unique carbon source through enrichment and domestication, screening, separation and purification, and reinforcement of acquired bacteria samples. The method can high efficiently and conveniently screen target active degradation bacteria, and provides strain resource for preparing related degradation bacteria agent and enzyme preparation.

Description

technical field [0001] The invention belongs to the field of biodegradation of pesticide residues, in particular to a screening method for degrading bacterial strains using lambda-cyhalothrin as a substrate. technical background [0002] The trade name of lambda-cyhalothrin is Kung Fu Chrysin, which belongs to a new type of pesticide containing fluorine in pyrethroid insecticides. Because it can also control mites, and the dosage is far lower than that of organophosphorus insecticides, it is very popular among farmers. [0003] While promoting the use of this type of pesticide in large quantities, its negative effects have also been found. According to my country's classification standards for pesticide toxicity, Kung Fu permethrin is a moderately toxic insecticide. Low toxicity to birds, highly toxic to bees, silkworms, fish and aquatic organisms. Studies have shown that Kung Fu pyrethrin can increase the osmotic fragility of carp erythrocytes, reduce the fluidity of ery...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04C12N1/02
Inventor 张惠文王秀娟李旭苏振成张晓黎张成刚
Owner 辽宁中科生物工程股份有限公司
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