Preparation of side-pocket mushroom cultispecies by adopting plastic breather bags

Abstract

The invention aims at providing a technology which adopts plastic breather bags to prepare side-pocket mushroom cultispecies. Plastic breather bags are used as the containers for formal culture; the side-pocket mushroom cultispecies are cultivated by the activated culture step of mother species, the pre-culture step for preparing protospecies and the formal culture step of inoculating the protospecies to the side-pocket cultispecies; and the preparation process adopts an improved autoclave to conduct sterilization and cooling treatments. By adopting the plastic breather bags and being combined with the special preparation steps and equipments, the invention is reliable, can fast produce side-pocket mushroom cultispecies in quantity, simultaneously can reduce the preparation cost, and lead the operation to achieve automation and save labor power; in the preparation process, the integration of autoclaving and aseptic cooling is realized, the quality of strain preparation is guaranteed, and the time cost and labor force are saved; the technology has simple and convenient operation, high yield and remarkable economic efficiency, and is easy for mechanized production.

Description

technical field [0001] The invention belongs to the field of microbiological technology and equipment thereof, and more specifically relates to a kind of mushroom cultivar produced in a large bag by using a breathing plastic bag. Background technique [0002] When carrying out the artificial cultivation of mushrooms (here usually referring to the white species, milk-colored species and brown species of Agaricus bisporus, the Latin scientific name Agaricusbisporus Langer), at first the cultivars must be made to ensure good harvests in future cultivation. my country is a big country in the production of Agaricus bisporus. The annual output of Agaricus bisporus in Fujian alone accounts for 10% of the world. The national demand for original species is 2-2.6 million bottles, cultivated species is 100-150 million bottles, and the output value is 200-300 million bottles. Yuan. The selection and breeding technology of Agaricus bisporus in Fujian Province has reached the internationa...

AI Technical Summary

Problems solved by technology

At present, the production process and equipment of mushroom strains are mostly in the state of manual workshops. The production process is relatively backward, and the production efficiency is low. However, the traditional strains use glass bottles, which have obvious defects: fragility, heavy weight, inconvenient transportation, and inconvenient use, etc., which restrict its development prospects

In terms of the use of plastic bags in China, the constraints of the bottleneck have not yet been overcome, and the two major problems of plastic strength and breathability have not been solved. Although the unit packaging cost has been reduced, the yield rate has declined, and large-scale application is also not possible.

The above factors make the high-quality strains selected and bred unable to be promoted better, and the quality of the cultivated species scattered in various places is not guaranteed, and a large number of unqualified strains are put into production every year, and the yield reduction is 10-15%. While the export of mushrooms is a big country, the export of mushroom strains is almost zero

[0003] In addition, conventional autoclaves cannot achieve rapid cooling under aseptic conditions after high-pressure sterilization of the culture medium, so that the quality of strain production cannot be guaranteed

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