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Nuclear acid probe marked with quantum dots and preparation method and application thereof

A technology of nucleic acid probes and quantum dots, which is applied in the fields of molecular biology and cytogenetics, can solve the problems of large interference between spectra and weak fluorescence intensity, and achieve strong fluorescence stability, stable probes, and reduced detection costs Effect

Inactive Publication Date: 2009-09-09
THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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Problems solved by technology

[0006] In view of this, one of the objects of the present invention is to provide a quantum dot-labeled nucleic acid probe, which can overcome the weak fluorescence intensity, easy photobleaching, and different fluorescent dye-labeled nucleic acid probes in the existing fluorescent dye-labeled nucleic acid probes. Defects such as needing different wavelengths for excitation and large interference between spectra

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  • Nuclear acid probe marked with quantum dots and preparation method and application thereof
  • Nuclear acid probe marked with quantum dots and preparation method and application thereof
  • Nuclear acid probe marked with quantum dots and preparation method and application thereof

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Embodiment Construction

[0027]The quantum dots used in the present invention mainly include single-core quantum dots (such as CdS, CdSe, CdTe, etc.) and quantum dots of core-shell structure (such as CdS / ZnO, CdSe / ZnS, CdTe / CdS, etc.). A quantum dot with a core-shell structure is a quantum dot composed of a material (such as CdSe, etc.) coated with another material with a larger energy band gap (such as CdS, ZnS, etc.), and its optical properties are determined by the core. , the outer shell can obviously improve the quantum yield and enhance the photochemical stability. In addition, it also includes quantum dots doped with transition metal elements (such as CdS:Mn, CdS:Cu, etc.) The spectral properties of quantum dots, and the construction of the silica shell can not only improve the fluorescence stability of quantum dots, facilitate the surface functionalization of quantum dots, but also help reduce the cytotoxicity caused by photolysis of quantum dots.

[0028] There are two main methods for the s...

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Abstract

The invention discloses a nuclear acid probe marked with quantum dots and a preparation method and application thereof. Probes with different sequences are marked with quantum dots with different emission spectrum characteristics; the preparation method of the probe has the following steps: synthesizing numerous probes with different sequences and marking the probes with X; synthesizing numerous quantum dots with different emission spectrum characteristics and marking the dots with Y; integrating probes marked with X and quantum dots marked with Y through specificity of X and Y and carrying out coupling on the probes and the quantum dots; facilitating probes with different sequences to be marked with quantum dots with different emission spectrum characteristics; the probes are used for multicolor fluorescence in situ hybridization, spectral karyotype analysis and chromosome karyotype analysis, can improve sensitivity, repetitiveness and specificity of detection, simplify detection device, lower detection cost, increase detection speed and prevent false positive result from occurring.

Description

technical field [0001] The invention relates to the fields of molecular biology and cytogenetics, in particular to a quantum dot labeled nucleic acid probe and its preparation method and application. Background technique [0002] Chromosomes of different species have their own specific morphological and structural characteristics (including chromosome length, centromere position, arm ratio, satellite size, etc.), and this morphological and structural characteristics are relatively stable. Therefore, karyotype analysis is an important content of genetic research, and it is also of great scientific significance for the study of the occurrence, development, and metastasis mechanisms of human chromosomal diseases and malignant tumors. [0003] The traditional method of chromosome karyotype analysis is mainly the chromosome G banding method, which has the advantages of simple operation and low cost, and can accurately distinguish the deletion, duplication and inversion of each nu...

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11C12N15/10G01N21/64
Inventor 罗阳王珏府伟灵黄君富
Owner THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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